Thomas E A, Cravatt B F, Danielson P E, Gilula N B, Sutcliffe J G
Department of Molecular Biology, The Scripps Research Institute, La Jolla, California 92037, USA.
J Neurosci Res. 1997 Dec 15;50(6):1047-52. doi: 10.1002/(SICI)1097-4547(19971215)50:6<1047::AID-JNR16>3.0.CO;2-1.
Fatty acid amide hydrolase (FAAH) is a membrane-bound enzyme activity that degrades neuromodulatory fatty acid amides, including oleamide and anandamide. A single 2.5-kb FAAH mRNA is distributed throughout the rat CNS and accumulates progressively between embryonic day 14 and postnatal day 10, remains high until postnatal day 30, then decreases into adulthood. FAAH enzymatic activity, as measured in dissected brain regions, was well correlated with the distribution of its messenger RNA. In situ hybridization revealed profound distribution of FAAH mRNA in neuronal cells throughout the CNS. The most prominent signals were detected in the neocortex, hippocampal formation, amygdala, and cerebellum. The FAAH distribution in the CNS suggests that degradation of neuromodulatory fatty acid amides at their sites of action influences their effects on sleep, euphoria, and analgesia.
脂肪酸酰胺水解酶（FAAH）是一种膜结合酶活性物质，可降解神经调节性脂肪酸酰胺，包括油酰胺和花生四烯乙醇胺。单一的2.5kb FAAH信使核糖核酸（mRNA）分布于整个大鼠中枢神经系统（CNS），并在胚胎第14天至出生后第10天逐渐积累，直至出生后第30天一直保持较高水平，然后在成年期下降。在解剖的脑区中测量的FAAH酶活性与其信使核糖核酸的分布密切相关。原位杂交显示FAAH mRNA在整个中枢神经系统的神经元细胞中广泛分布。在新皮层、海马结构、杏仁核和小脑中检测到最显著的信号。FAAH在中枢神经系统中的分布表明，神经调节性脂肪酸酰胺在其作用部位的降解会影响它们对睡眠、欣快感和镇痛的作用。
