Baba K, Ikeda M, Houtani T, Nakagawa H, Ueyama T, Sato K, Sakuma S, Yamashita T, Tsukahara Y, Sugimoto T
Department of Anatomy, Kansai Medical University, Moriguchi, Osaka, Japan.
Brain Res. 1997 Nov 7;774(1-2):142-8. doi: 10.1016/s0006-8993(97)81697-6.
In the main olfactory bulb, neurons are arranged strategically in distinct layers among which translaminar synaptic transmission can be made from the superficial, sensory to the deep, output layers that account for the processing of olfactory information. To search for stimulus-transcription coupling thought to be operated differentially in several cell types, c-Jun expression was examined immunohistochemically in rat olfactory bulb following 30-min odor stimulation with acetic acid and 1-butanol. c-Jun was rapidly induced in neuronal cell nuclei belonging to periglomerular, tufted, mitral and granule cells. The disappearance of c-Jun, however, differed between each cell type. In the glomerular layer, the glomeruli composed of c-Jun-expressing periglomerular cells were seen. Different odors led to labeling of different sets of glomeruli. The labeled periglomerular cells disappeared within 2 h. In all the deeper layers, however, a rather homogeneous label was noted for the tufted, mitral and granule cells present throughout the olfactory bulb, regardless of the difference in odor. In tufted and mitral cells, the c-Jun expression persisted for 4 days after odor stimulation. In the granule cell layer, numerous granule cells increased c-Jun immunoreactivity which lasted for 1 day following odor application. In control rats which were given clean air, the basal amount of c-Jun expression was seen confined to scattered granule cells. The results suggest that c-Jun is expressed in a variety of odorant-stimulated bulb neurons with a time course being dependent on cell type.
在主嗅球中，神经元按特定方式排列在不同的层中，在这些层之间可进行跨层突触传递，即从浅层的感觉层传递至深层的输出层，这一过程负责嗅觉信息的处理。为了寻找被认为在几种细胞类型中差异运作的刺激-转录偶联，在用乙酸和1-丁醇进行30分钟气味刺激后，通过免疫组织化学方法检测了大鼠嗅球中c-Jun的表达。c-Jun在属于球周细胞、簇状细胞、僧帽细胞和颗粒细胞的神经元细胞核中迅速被诱导表达。然而，c-Jun的消失在每种细胞类型中有所不同。在嗅小球层，可以看到由表达c-Jun的球周细胞组成的嗅小球。不同的气味导致不同组别的嗅小球被标记。被标记的球周细胞在2小时内消失。然而，在所有更深的层中，无论气味差异如何，在整个嗅球中存在的簇状细胞、僧帽细胞和颗粒细胞都呈现出相当均匀的标记。在簇状细胞和僧帽细胞中，气味刺激后c-Jun表达持续4天。在颗粒细胞层，大量颗粒细胞的c-Jun免疫反应性增加，在施加气味后持续1天。在给予清洁空气的对照大鼠中，c-Jun表达的基础量局限于散在的颗粒细胞中。结果表明，c-Jun在多种受气味刺激的嗅球神经元中表达，其时间进程取决于细胞类型。
