Nakahara K, Murakami N, Nasu T, Kuroda H, Murakami T
Department of Veterinary Physiology, Faculty of Agriculture, University of Miyazaki, Japan.
Brain Res. 1997 Nov 7;774(1-2):242-5. doi: 10.1016/s0006-8993(97)81713-1.
Chick pineal cells express a circadian rhythm of melatonin release under light-dark (LD) cycles, with an increase during the dark period and a decrease during the light period, and this rhythm persists under constant darkness (DD). We cultured individual single pineal cells with 15 microl of medium per well in a Terasaki plate and measured melatonin secretion every 12 h under LD, DL and DD. Individual cells secreted more melatonin during the dark period than during the light period under both LD and DL conditions, and those rhythmic secretions persisted under DD. These results suggest that individual pineal cells in chick have photoreceptive, circadian clock and melatonin-synthesizing capacities.
鸡松果体细胞在明暗（LD）循环下表现出褪黑素释放的昼夜节律，黑暗期增加而光照期减少，并且这种节律在持续黑暗（DD）条件下依然存在。我们在培养皿中每孔用15微升培养基培养单个松果体细胞，并在LD、DL和DD条件下每12小时测量一次褪黑素分泌。在LD和DL条件下，单个细胞在黑暗期比光照期分泌更多的褪黑素，并且这些节律性分泌在DD条件下持续存在。这些结果表明，鸡的单个松果体细胞具有光感受、昼夜节律钟和褪黑素合成能力。
