Raviprakash K, Sinha M, Hayes C G, Porter K R
Infectious Diseases Department, Naval Medical Research Institute, Bethesda, Maryland 20889, USA.
Am J Trop Med Hyg. 1998 Jan;58(1):90-5. doi: 10.4269/ajtmh.1998.58.90.
Dengue viruses infect more than 100 million people each year and cause serious clinical manifestations. It is important to understand the replication of these viruses so that therapeutic and/or prophylactic agents may be designed. Dengue virus type 2 nonstructural proteins NS-5 and NS-3 were produced by in vitro transcription and translation of cloned genes. Both proteins possessed RNA-dependent RNA polymerase activity as measured by their ability to convert purified replicative form (RF) RNA to replicative intermediate (RI). The recombinant proteins, however, required one or more cellular protein(s) for their activity. Examination of NS-3 protein sequence revealed heretofore unnoticed sequence similarities with other polymerases.
登革病毒每年感染超过1亿人，并引发严重的临床表现。了解这些病毒的复制过程对于设计治疗和/或预防药物至关重要。通过对克隆基因进行体外转录和翻译，制备了登革2型病毒的非结构蛋白NS-5和NS-3。通过它们将纯化的复制型（RF）RNA转化为复制中间体（RI）的能力测定，这两种蛋白均具有RNA依赖性RNA聚合酶活性。然而，重组蛋白的活性需要一种或多种细胞蛋白。对NS-3蛋白序列的研究揭示了此前未被注意到的与其他聚合酶的序列相似性。
