Huang L, Grammatikakis N, Toole B P
Department of Anatomy and Cellular Biology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
J Biol Chem. 1998 Feb 6;273(6):3598-603. doi: 10.1074/jbc.273.6.3598.
CDC37 and the chaperone protein, Hsp90, form a complex that binds to several kinases, resulting in stabilization and promotion of their activity. CDC37 also binds DNA and glycosaminoglycans in a sequence-specific manner. In this study, we further characterize chick CDC37 and examine the organization of the CDC37 gene. Chick CDC37 is a approximately 50-kDa protein encoded by an mRNA of approximately 1.7 kilobases. The CDC37 gene is approximately 8.5 kilobases and contains 8 exons and 7 introns of various sizes. The presumptive promoter and 5'-flanking regions contain an E2 box and consensus binding sites for SP1, for the S8 homeodomain protein, and for two zinc finger clusters within the myeloid progenitor transcription factor, MZF1. Particularly striking is a approximately 470-base pair region composed of a highly repetitive 10-11-base pair sequence, (T/C)gCTAT(A/G)GGG(A/T) (where g represents the additional G present in the 11-base pair sequence). This region includes 15 copies of the sequence, TATGGGGA, which conforms to the DNA consensus sequence recognized by one of the zinc finger clusters in MZF1. These findings emphasize the potential importance of CDC37 in regulation of cellular behavior during tissue development and reorganization.
CDC37与伴侣蛋白Hsp90形成复合物，该复合物可与多种激酶结合，从而稳定并促进其活性。CDC37还能以序列特异性方式结合DNA和糖胺聚糖。在本研究中，我们进一步对鸡CDC37进行了表征，并研究了CDC37基因的组织结构。鸡CDC37是一种约50 kDa的蛋白质，由一个约1.7千碱基的mRNA编码。CDC37基因约为8.5千碱基，包含8个外显子和7个大小各异的内含子。推测的启动子和5'侧翼区域包含一个E2框以及SP1、S8同源域蛋白和髓系祖细胞转录因子MZF1内两个锌指簇的共有结合位点。特别引人注目的是一个约470碱基对的区域，由高度重复的10 - 11碱基对序列（T/C）gCTAT（A/G）GGG（A/T）组成（其中g代表11碱基对序列中额外的G）。该区域包含15个TATGGGGA序列拷贝，其符合MZF1中一个锌指簇识别的DNA共有序列。这些发现强调了CDC37在组织发育和重组过程中调节细胞行为方面的潜在重要性。
