Turina M, Maruoka M, Monis J, Jackson A O, Scholthof K B
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas, 77843, USA.
Virology. 1998 Feb 1;241(1):141-55. doi: 10.1006/viro.1997.8939.
The sequence of an infectious cDNA clone of panicum mosaic virus (PMV) showed that the single-stranded RNA genome is 4326 nucleotides (nt) and a single highly abundant subgenomic (sg) RNA of 1475 nt was synthesized during PMV infection of pearl millet plants and protoplasts. Computer comparisons revealed strong similarities between the predicted amino acid sequences of the p48 and p112 open reading frames (ORFs) and replicase proteins of members of the Tombusviridae. The sgRNA has the potential to encode five proteins. Three small ORFs, p8, p8-FS, and/or p6.6 have similarity to ORFs of carmo-, necro-, and machlomoviruses thought to be involved in virus spread in plants. The sgRNA also has the potential to encode a 26-kDa capsid protein and a 15-kDa nested gene (p15) of unknown function. PMV transcripts also supported replication and movement of SPMV, the satellite virus. Genome organization, physicochemical properties, and biological features indicate that PMV is a member of the Tombusviridae family. However, PMV differs sufficiently from previously described members to warrant its placement in a new genus provisionally designated Panicovirus.
黍花叶病毒（PMV）感染性cDNA克隆的序列显示，其单链RNA基因组为4326个核苷酸（nt），在珍珠粟植株和原生质体受到PMV感染期间，合成了一条1475 nt的高度丰富的单亚基因组（sg）RNA。计算机比对显示，p48和p112开放阅读框（ORF）的预测氨基酸序列与番茄病毒科成员的复制酶蛋白有很强的相似性。该sgRNA有编码5种蛋白质的潜力。三个小ORF，即p8、p8-FS和/或p6.6，与被认为参与病毒在植物中传播的香石竹潜隐病毒属、坏死病毒属和玉米褪绿斑驳病毒属的ORF相似。该sgRNA还有编码一种26 kDa衣壳蛋白和一个功能未知的15 kDa嵌套基因（p15）的潜力。PMV转录本也支持卫星病毒SPMV的复制和移动。基因组结构、理化特性和生物学特征表明，PMV是番茄病毒科的成员。然而，PMV与先前描述的成员有足够的差异，因此有必要将其暂时归入一个新属，即暂定为黍病毒属。
