Schoeberl C, Boehner R, Krebs B, Mueller C, Barnekow A
Department of Experimental Tumor Biology, University of Münster, Badestr. 9, 48149 Münster, Germany.
Int J Oncol. 1998 Jan;12(1):153-9.
We examined the recently synthesized and characterized polyoxometalate compound (NH4)10[Co2Sb2W20 O70(H2O)6] (POM1). The inhibitory potency of POM1 was studied in tissue culture experiments with uninfected and Rous sarcoma virus (RSV)-infected chicken embryo fibroblasts (CEF) in vivo. We measured a considerable decrease in total cellular phosphotyrosine content in treated infected cells in vivo. POM1 treatment of SR-RSV-A infected CEF in vivo resulted in decreased pp60v-src activity, possibly due to a reduced rate of v-src translation caused by the inhibitory effect of POM1 on the RSV encoded reverse transcriptase activity (RT) activity. In further studies we were able to demonstrate the inhibitory effect of this complex on the RT activity of RSV in vitro and in vivo.
我们研究了最近合成并表征的多金属氧酸盐化合物(NH4)10Co2Sb2W20O70(H2O)6。在体内对未感染和感染劳氏肉瘤病毒(RSV)的鸡胚成纤维细胞(CEF)进行的组织培养实验中，研究了POM1的抑制效力。我们测量到体内经处理的感染细胞中总细胞磷酸酪氨酸含量有显著下降。在体内用POM1处理感染SR-RSV-A的CEF导致pp60v-src活性降低，这可能是由于POM1对RSV编码的逆转录酶活性(RT)的抑制作用导致v-src翻译速率降低。在进一步的研究中，我们能够证明这种复合物在体外和体内对RSV的RT活性均有抑制作用。
