Meding J H
Nord Vet Med. 1976 Apr-May;28(4-5):221-5.
Ejaculates from six boars of Danish Landrace were divided into four parts, I, II, III and IV which were treated in the following way: I was diluted 1:4-1:6 with EDTA-diluter, and filled into glass vials (single doses). II was centrifugated at 1800-2000 r.p.m. in 12-15 min.; the centifugate was resuspended in the supernatant and diluted as indicated under I. III and IV were centrifugated at 1800-2000 r.p.m. in 12-15 min. The supernatant was removed by aspiration and the centrifugate diluted with as much EDTA-diluter necessary to produce the same no, of doses of 10 ml as was produced from I and II respectively. The diluted semen was stored at 18-20 degrees C and used on the day of collection. Just before insemination the semen from I, II and III was diluted with IVT-diluter to a final volume of 75 ml. Semen from IV was diluted to the same volume with IVT-diluter with the addition of 50% boar seminal plasma, procured by centrifugation of ejaculates from other boars of the A.I. Centre, and stored at divided by 20 degrees C until use. The results have been set out in Table I. Inseminations with semen from I and II resulted in pregnancy rates of 84,6% and 86,3% and average litter sizes of 10,3 and 10,1 for sows and 9,1 and 9,6 for gilts; inseminations with semen from III and IV resulted in pregnancy rates of 78,9 and 78,2% and average litter sizes of 10,5 and 10,7 for sows and 10,0 and 8,7 for gilts. None of the differences in pregnancy rates between groups were significant. The findings were: 1. By insemination with initially diluted semen without seminal plasma a tendency to a decline in pregnancy rates could be demonstrated. 2. The decline persisted after the addition of 50% boar seminal plasma to the diluter used for the final dilution just before insemination.
来自六头丹麦长白公猪的射精精液被分成四部分，即I、II、III和IV，处理方式如下：I用EDTA稀释液按1:4至1:6的比例稀释，然后装入玻璃瓶（单剂量）。II在1800 - 2000转/分钟的转速下离心12 - 15分钟；将离心沉淀物重新悬浮于上清液中，并按I中所示进行稀释。III和IV在1800 - 2000转/分钟的转速下离心12 - 15分钟。通过抽吸去除上清液，并用所需量的EDTA稀释液稀释离心沉淀物，使其产生与I和II分别产生的相同数量的10毫升剂量。稀释后的精液在18 - 20摄氏度下储存，并在采集当天使用。就在输精前，将来自I、II和III的精液用IVT稀释液稀释至最终体积75毫升。来自IV的精液用IVT稀释液稀释至相同体积，并添加50%的公猪精浆，该精浆是通过对人工授精中心其他公猪的射精精液进行离心获得的，并在20摄氏度下分装储存直至使用。结果列于表I中。用来自I和II的精液输精，母猪的妊娠率分别为84.6%和86.3%，平均产仔数分别为10.3和10.1；后备母猪的妊娠率分别为91%和96%，平均产仔数分别为9.1和9.6。用来自III和IV的精液输精，母猪的妊娠率分别为78.9%和78.2%，平均产仔数分别为10.5和10.7；后备母猪的妊娠率分别为100%和87%，平均产仔数分别为10.0和8.7。各组之间妊娠率的差异均无统计学意义。研究结果如下：1. 通过使用最初稀释且无精浆的精液进行输精，可以证明妊娠率有下降趋势。2. 在输精前用于最终稀释的稀释液中添加50%的公猪精浆后，这种下降趋势仍然存在。
