Pfau G, Westphal S, Dietzmann K, von Bossanyi P, Augustin W
Department of Neuropathology, Medical School, Otto-von-Guericke-University Magdeburg, Germany.
Exp Toxicol Pathol. 1997 Dec;49(5):361-7. doi: 10.1016/s0940-2993(97)80115-7.
The aim of the study was to analyse the intrauterine effects of Chloroquine on the dendritic maturation in the hippocampal region under considering of the lipid composition in brain tissue. 131 brains of rat pups from day 22 of pregnancy and 49 brains from offsprings from day 7 of life were investigated. The findings indicate changes in the geometric dendritic architecture of the CA3 neurons on the 7th day of life. The length of apical shaft, apical dendrites and basilar dendrites of the CA3 neurons showed a significant elongation (p < 0.05) under low doses of Chloroquine. Furthermore a early considerable formation of dendritic spines during the intrauterine period could be observed for CA1 spines at the day 22 p.c. A significant increase of the fatty acids, a reduced amount of sphingomyelines, cephalines and gangliosides was found. These results underline the fact of a mild toxic effect under a low dosis of Chloroquine in our model.
本研究的目的是在考虑脑组织脂质组成的情况下，分析氯喹对海马区树突成熟的宫内影响。对妊娠第22天的131只大鼠幼崽的大脑和出生后第7天的49只后代的大脑进行了研究。研究结果表明，出生后第7天CA3神经元的几何树突结构发生了变化。低剂量氯喹作用下，CA3神经元顶轴、顶树突和基底树突的长度显著延长（p<0.05）。此外，在妊娠第22天，可观察到CA1树突棘在宫内期早期大量形成。研究发现脂肪酸显著增加，鞘磷脂、脑磷脂和神经节苷脂的含量减少。这些结果强调了在我们的模型中低剂量氯喹存在轻度毒性作用这一事实。
