Taranenko A G, Marinchenko G V
Probl Endokrinol (Mosk). 1976 May-Jun;22(3):61-6.
A study was made of the dynamics of prolactin-I125 accumulation in the kidneys, mammary gland and the liver of lactating rats in the course of 100 min. after the administration of the hormone. Absorption of the labeled prolactin occurred most intensively in the kidneys, where it splits. Iodides served as the principal radioactive product of prolactin-I125 splitting in the urine. The liver displayed no significant prolactin-binding activity. Prolactin-I125 accumulation in the mammary gland occurred at a constant rate. In this tissue labeled prolactin was localized outside the cells -- at first in the lymphatic and intercellular space and then -- in milk. The interval from the time of the hormone accumulation in the tissue to the appearance of radioactive products in milk was about 13 minutes. Prolactin-binding fragments of plasmatic membranes of the mammary gland cells were distributed in fractionation of this tissue by the method of differential centrifugation in the mitochondrial, lysosomal and microsomal fractions.
对泌乳大鼠在注射激素后100分钟内肾脏、乳腺和肝脏中催乳素-I125积累的动力学进行了研究。标记催乳素的吸收在肾脏中最为强烈，它在那里分解。碘化物是尿液中催乳素-I125分解的主要放射性产物。肝脏未显示出明显的催乳素结合活性。催乳素-I125在乳腺中以恒定速率积累。在该组织中，标记的催乳素定位于细胞外——起初在淋巴和细胞间隙，然后——在乳汁中。从激素在组织中积累到乳汁中出现放射性产物的间隔约为13分钟。通过差速离心法对乳腺细胞进行分级分离时，乳腺细胞质膜的催乳素结合片段分布在线粒体、溶酶体和微粒体组分中。
