使用毛细管电泳进行氨基酸分析的进展。
Developments in amino acid analysis using capillary electrophoresis.
作者信息
Smith J T
机构信息
Department of Physical Sciences, Southeastern Oklahoma State University, Durant 74701-0609, USA.
出版信息
Electrophoresis. 1997 Nov;18(12-13):2377-92. doi: 10.1002/elps.1150181228.
The current status in the analysis of amino acids using capillary electrophoresis is addressed. This area of biological analysis has received increased attention with more than 200 articles being published in the last five years. This review discusses pre-, post-, and on-column derivatization techniques used to tag amino acids providing a detectable moiety. Several separation methodologies which provided resolution for large sets of amino acids are presented. An overview of advances in the enantiomeric resolution methodologies for amino acids is given. Both direct and indirect enantiomeric separation schemes are summarized. Recent advances in detection strategies for both derivatized and underivatized amino acids are presented. Applications utilizing amino acid analysis by capillary electrophoresis are described. This review covers articles published between 1991 and 1996.
本文论述了利用毛细管电泳分析氨基酸的现状。在过去五年里，这一生物分析领域受到了越来越多的关注，发表的文章超过200篇。本综述讨论了用于标记氨基酸以提供可检测部分的柱前、柱后和柱上衍生化技术。介绍了几种能够分离大量氨基酸的分离方法。给出了氨基酸对映体拆分方法的进展概述。总结了直接和间接对映体分离方案。介绍了衍生化和未衍生化氨基酸检测策略的最新进展。描述了利用毛细管电泳进行氨基酸分析的应用。本综述涵盖了1991年至1996年发表的文章。
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