[银染法对人睫状神经营养因子基因的DNA测序]
[DNA sequencing of human ciliary neurotropic factor gene by silver staining method].
作者信息
Fu X, Huang X, Gao Y, Hu X
机构信息
Institute of Medical Sciences, Nanjing Railway Medical College, Nanjing, 210009 P. R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1998 Feb 10;15(1):49-50.
OBJECTIVE
To introduce a non-radioactive protocol for DNA sequence analysis which employs a silver staining procedure
METHODS
DNA template is amplified by Taq DNA polymerase to generate sequence ladder, No labeled nucleotide or primer is involved in the thermal-cycling sequencing reaction. A sensitive silver staining procedure is employed to visualize bands in sequencing gel.
RESULTS
This silver staining method is used for DNA sequence analysis of human cilliary neurotrophic factor (hCNTF) gene. The resulted band resolution is comparable to radioactive sequencing method, and data can be obtain within less than 2 hours after sequencing reaction.
CONCLUSION
Silver staining is a rapid, inexpensive and reliable method for DNA sequence analysis.
目的
介绍一种采用银染程序进行DNA序列分析的非放射性方法。
方法
用Taq DNA聚合酶扩增DNA模板以生成序列梯，热循环测序反应中不涉及标记核苷酸或引物。采用灵敏的银染程序使测序凝胶中的条带可视化。
结果
该银染方法用于人睫状神经营养因子（hCNTF）基因的DNA序列分析。所得条带分辨率与放射性测序方法相当，测序反应后不到2小时即可获得数据。
结论
银染是一种用于DNA序列分析的快速、廉价且可靠的方法。
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