Mattia E, Eufemi M, Chichiarelli S, Ceridono M, Ferraro A
Microbiology Institute Faculty of Medicine and Surgery, University of Rome La Sapienza, Italy.
Exp Cell Res. 1998 Jan 10;238(1):216-9. doi: 10.1006/excr.1997.3833.
DNA-protein cross-linkages were performed in intact undifferentiated and differentiated-HL60 cells by the action of cis-diammine dichloroplatinum. Total nuclear matrix proteins and DNA cross-linked nuclear matrix proteins were resolved by two-dimensional gel electrophoresis. The comparison of the electrophoretic patterns allowed the identification of a set of differentiation-induced nuclear matrix proteins cross-linked to DNA. One of these proteins binds cloned histone SAR sequences. Our results outline an experimental strategy for isolating and characterizing nuclear matrix components that may play a fundamental role in the overall control and coordination of gene expression during differentiation.
通过顺二氯二氨铂的作用，在完整的未分化和已分化的HL60细胞中形成DNA-蛋白质交联。通过二维凝胶电泳解析总核基质蛋白和与DNA交联的核基质蛋白。电泳图谱的比较使得能够鉴定出一组与DNA交联的分化诱导核基质蛋白。其中一种蛋白结合克隆的组蛋白SAR序列。我们的结果概述了一种实验策略，用于分离和表征在分化过程中可能在基因表达的整体控制和协调中起基本作用的核基质成分。
