Moser V C, Becking G C, Cuomo V, Frantík E, Kulig B M, MacPhail R C, Tilson H A, Winneke G, Brightwell W S, De Salvia M A, Gill M W, Haggerty G C, Hornychová M, Lammers J, Larsen J J, McDaniel K L, Nelson B K, Ostergaard G
Neurotoxicology Division, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711 USA.
Neurotoxicology. 1997;18(4):969-1055.
The IPCS Collaborative Study on Neurobehavioral Screening Methods was undertaken to determine the intra- and inter-laboratory reliability of a functional observational battery (FOB) and an automated assessment of motor activity in eight laboratories world-wide. Following the training phase and the conduct of proficiency studies in all laboratories, participants proceeded to test the effects of seven chemicals in both single dose and four-week repeated dosing scenarios. The chemicals studied were acrylamide, bisacrylamide, p,p'-DDT, lead acetate, parathion, toluene, and triethyl tin. Participants received coded samples from a common source. In order to judge the general utility of these procedures in a diversity of testing situations, laboratories conducted the studies under their standard conditions, using their choice of rat strain and test equipment. Chemical does and time of peak effect for acute testing were determined by each laboratory: these parameters were quite similar for some chemicals, but varied greatly for others. The results of the chemical tests indicated that while there was some variability in the data on specific endpoints, all laboratories detected and characterized the effects of all but one of the known neurotoxicants. The one exception (toluene) was probably due to other factors (e.g., dose level, route of administration) rather than lack of sensitivity of the test methods. This study provides extensive data regarding the use of neurobehavioral screening methods over a range of laboratory conditions as well as the reliability, sensitivity, and robustness of the tests to detect neurotoxic potential of chemicals.
国际化学品安全规划署（IPCS）开展了神经行为筛查方法合作研究，以确定全球八个实验室中功能观察组合测试（FOB）和运动活动自动评估的实验室内和实验室间可靠性。在所有实验室完成培训阶段和能力验证研究后，参与者着手测试七种化学品在单剂量和四周重复给药情况下的效果。所研究的化学品有丙烯酰胺、双丙烯酰胺、p,p'-滴滴涕、醋酸铅、对硫磷、甲苯和三乙基锡。参与者收到来自同一来源的编码样本。为了判断这些程序在各种测试情况下的普遍适用性，各实验室在其标准条件下进行研究，使用各自选择的大鼠品系和测试设备。各实验室自行确定急性测试的化学剂量和峰值效应时间：这些参数对某些化学品非常相似，但对其他化学品则差异很大。化学测试结果表明，虽然特定终点数据存在一定变异性，但所有实验室都检测并描述了除一种已知神经毒物外的所有毒物的影响。唯一的例外（甲苯）可能是由于其他因素（如剂量水平、给药途径），而非测试方法缺乏敏感性。本研究提供了关于在一系列实验室条件下使用神经行为筛查方法的数据，以及这些测试检测化学品神经毒性潜力的可靠性、敏感性和稳健性。
