一种用于HLA-C等位基因以及日本人和高加索人之间可能共享的HLA-C-B单倍型的高分辨率基因分型方法。

A high-resolution genotyping method for HLA-C alleles and possible shared HLA-C-B haplotypes between Japanese and Caucasians.

作者信息

Wang H, Tokunaga K, Ishikawa Y, Tanaka H, Kashiwase K, Shibata Y, Juji T

机构信息

Department of Research, Japanese Red Cross Central Blood Center, Tokyo, Japan.

出版信息

Tissue Antigens. 1997 Dec;50(6):620-6. doi: 10.1111/j.1399-0039.1997.tb02920.x.

DOI:10.1111/j.1399-0039.1997.tb02920.x

PMID:9458115

Abstract

A genotyping system for HLA-Cw alleles using methods of PCR-single-strand conformation polymorphism (SSCP) and PCR-sequence-specific primers (SSP) was developed. Genomic DNA from 167 random healthy Japanese individuals were investigated to determine HLA-C allele frequencies and their associations with HLA-A and -B loci. Nine alleles were frequently detected: Cw0102 (18.3%), Cw0304 (12.0%), Cw1202 (11.4%), Cw0303 (10.5%), Cw1403 (10.5%), Cw0801 (10.2%), Cw0702 (9.0%), Cw1402 (6.0%) and Cw0401 (5.1%). Several HLA-C-B haplotypes such as Cw0303-B62, Cw0304-B60, Cw0501-B44, Cw0602-B13, Cw0602-B37, Cw0702-B7, Cw1202-B52, Cw1402-B51 and Cw1502-B51 were found to be shared by Japanese and Caucasians. With the PCR-SSCP method we could detect differences in each exon of HLA-C alleles, at a low cost and simply. This method is suitable for sequence-level matching with a relatively small number of samples.

摘要

开发了一种使用聚合酶链反应-单链构象多态性（PCR-SSCP）和聚合酶链反应-序列特异性引物（PCR-SSP）方法的HLA-Cw等位基因基因分型系统。对167名随机选取的健康日本个体的基因组DNA进行研究，以确定HLA-C等位基因频率及其与HLA-A和-B位点的关联。经常检测到9个等位基因：Cw0102（18.3%）、Cw0304（12.0%）、Cw1202（11.4%）、Cw0303（10.5%）、Cw1403（10.5%）、Cw0801（10.2%）、Cw0702（9.0%）、Cw1402（6.0%）和Cw0401（5.1%）。发现几种HLA-C-B单倍型，如Cw0303-B62、Cw0304-B60、Cw0501-B44、Cw0602-B13、Cw0602-B37、Cw0702-B7、Cw1202-B52、Cw1402-B51和Cw1502-B51在日本人和高加索人中是共有的。使用PCR-SSCP方法，我们可以以低成本且简单地检测HLA-C等位基因每个外显子的差异。该方法适用于对相对少量样本进行序列水平匹配。