Romiti A, Moretti A, Vecchione A, Muraro R, Feudi M L, Rinaldi V, Mancini R, Valli C, Mozzicafreddo A, Frati L, Tomao S
Istituto Nazionale per la Ricerca sul Cancro di Genova Sezione di Biotecnologie, Roma, 00171, Italy.
Oncol Rep. 1998 Jan-Feb;5(1):109-13.
P53 overexpression, detected by immunohistochemical analysis, has been reported in about 50% of gastric cancers whereas scarce data are available on the p53 oncoprotein in precancerous gastric lesions. This study focused on the p53 expression in gastric cancerous and precancerous lesions. One hundred gastric specimens obtained during endoscopy were analyzed: 14 cases of normal gastric mucosa, 53 of chronic gastritis with intestinal metaplasia and/or dysplasia and 33 gastric tumors. An immunoperoxidase technique and monoclonal anti-p53 antibodies were employed. Eleven out of 31 gastric carcinomas overexpressed p53. No correlation was observed between p53-positivity and histological type and grade of tumors. All precancerous lesions were p53-negative. Our results suggest that p53 overexpression is a relatively late event in gastric carcinogenesis.
通过免疫组织化学分析检测到，约50%的胃癌中存在p53过表达，而关于癌前胃病变中p53癌蛋白的数据却很少。本研究聚焦于胃癌和癌前病变中的p53表达。对在内镜检查期间获取的100份胃标本进行了分析：14例正常胃黏膜、53例伴有肠化生和/或发育异常的慢性胃炎以及33例胃肿瘤。采用了免疫过氧化物酶技术和单克隆抗p53抗体。31例胃癌中有11例p53过表达。未观察到p53阳性与肿瘤的组织学类型和分级之间存在相关性。所有癌前病变均为p53阴性。我们的结果表明，p53过表达是胃癌发生过程中相对较晚出现的事件。
