Takamiya O, Kinoshita S
Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Asahi, Japan.
Scand J Clin Lab Invest. 1997 Dec;57(8):683-8. doi: 10.3109/00365519709105229.
We developed a simple ELISA for FIX inhibitors (FIXinh). The relationship between absorbance and various dilutions of plasma containing known FIXinh (28 Bethesda units/ml) was linear, ranging from 200 to 25,600-fold dilution on semi-log paper. Converted into the value of Bethesda Inhibitor Assay, ELISA can detect as low as 0.219 Bethesda units/ml. Absorbance for 30 normal individuals was 0.213 +/- 0.008, which corresponds to < 0.44 Bethesda units/ml. With ELISA, only the value for hemophilia B with FIXinh, which was revealed as positive by Bethesda Inhibitor Assay, was significantly higher than that of normal controls, hemophilia B without FIXinh, hemophilia A with FVIIIinh and systematic lupus erythematosus (SLE) with lupus anticoagulant (LA). The value in the patient with SLE, who had a high level of FVIIIinh (1000 Bethesda units/ml), was 3 Bethesda units/ml of FIXinh with Bethesda Inhibitor Assay, which was not significantly higher than that of normal subjects with ELISA. FIXinh in hemophilia B patients by ELISA for FIXinh was not affected by the presence of other anticoagulants.
我们开发了一种用于检测FIX抑制物（FIXinh）的简易酶联免疫吸附测定法（ELISA）。在半对数纸上，吸光度与含有已知FIX抑制物（28贝塞斯达单位/毫升）的血浆不同稀释度之间呈线性关系，稀释范围为200至25,600倍。换算为贝塞斯达抑制物测定值后，ELISA可检测低至0.219贝塞斯达单位/毫升的FIX抑制物。30名正常个体的吸光度为0.213±0.008，相当于<0.44贝塞斯达单位/毫升。采用ELISA法时，只有经贝塞斯达抑制物测定显示为阳性的B型血友病伴FIX抑制物患者的值显著高于正常对照、无FIX抑制物的B型血友病、伴FVIII抑制物的A型血友病以及伴狼疮抗凝物（LA）的系统性红斑狼疮（SLE）患者。SLE患者中FVIII抑制物水平较高（1000贝塞斯达单位/毫升），其经贝塞斯达抑制物测定的FIX抑制物值为3贝塞斯达单位/毫升，采用ELISA法时该值与正常受试者相比无显著差异。通过ELISA法检测B型血友病患者的FIX抑制物不受其他抗凝剂存在的影响。
