Shkidchenko A N
Mikrobiologiia. 1976 Jan-Feb;45(1):67-72.
The cells of Candida utilis were cultivated in the conditions of chemostat, and the growth was limited by a nitrogen source. Accumulation in the medium of carbon dioxide inhibited the growth of the microbial population. The growth was now limited not by a source of nitrogen but by an excess of one of the forms of carbon dioxide--hydrocarbonate. This inhibition was reversible and could be eliminated either by intensifying ventilation of the apparatus or by varying pH of the medium within the range optimal for the growth, which changed the ratio between free and hydrocarbonate forms of carbon dioxide in the growth medium.
产朊假丝酵母细胞在恒化器条件下培养,生长受氮源限制。培养基中二氧化碳的积累抑制了微生物群体的生长。现在生长不是受氮源限制,而是受一种二氧化碳形式——碳酸氢盐过量的限制。这种抑制是可逆的,可以通过加强仪器通风或在生长最适宜的pH范围内改变培养基的pH来消除,这会改变生长培养基中游离态和碳酸氢盐态二氧化碳的比例。