不育男性精子中雌激素和孕激素受体的免疫组织化学检测

Immunohistochemical detection of estrogen and progesterone receptors in spermatozoa of infertile men.

作者信息

Misao R, Niwa K, Morishita S, Fujimoto J, Nakanishi Y, Tamaya T

机构信息

Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.

出版信息

Int J Fertil Womens Med. 1997 Nov-Dec;42(6):421-5.

PMID:9459087

Abstract

OBJECTIVE

To evaluate the clinical implication of sex steroidal actions on human spermatozoa.

PATIENTS AND METHODS

Human spermatozoa were obtained from 20 males. Immunohistochemical staining for estrogen receptors (ER) and progesterone receptors (PR) was conducted by the avidin-biotin-peroxidase complex method.

RESULTS

Positive immunohistochemical staining of ER and PR was observed in all 8 cases of fertile couples. From infertile couples, ER were detected in 8/12 cases, including 3 with normozoospermia, 2 with oligozoospermia, 1 with asthenozoospermia, and 2 with teratozoospermia, but not in 4/12 cases, 1 with normozoospermia, 1 with asthenozoospermia, and 2 with oligoasthenozoospermia. PR were undetectable in all cases of negative ER and in 1 case (normozoospermial) of positive ER.

CONCLUSION

Negative ER and/or PR in spermatozoa from infertile couples might be involved in cases of male infertility.

摘要

目的

评估性甾体激素对人类精子作用的临床意义。

患者与方法

从20名男性获取人类精子。采用抗生物素蛋白-生物素-过氧化物酶复合物法进行雌激素受体（ER）和孕激素受体（PR）的免疫组织化学染色。

结果

在所有8对可育夫妇的样本中均观察到ER和PR的阳性免疫组织化学染色。在不育夫妇的样本中，12例中有8例检测到ER，其中3例为正常精子症，2例为少精子症，1例为弱精子症，2例为畸形精子症；但12例中的4例未检测到ER，其中1例为正常精子症，1例为弱精子症，2例为少弱精子症。在所有ER阴性的病例以及1例ER阳性（正常精子症）病例中均未检测到PR。