Misao R, Niwa K, Morishita S, Fujimoto J, Nakanishi Y, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Japan.
Int J Fertil Womens Med. 1997 Nov-Dec;42(6):421-5.
To evaluate the clinical implication of sex steroidal actions on human spermatozoa.
Human spermatozoa were obtained from 20 males. Immunohistochemical staining for estrogen receptors (ER) and progesterone receptors (PR) was conducted by the avidin-biotin-peroxidase complex method.
Positive immunohistochemical staining of ER and PR was observed in all 8 cases of fertile couples. From infertile couples, ER were detected in 8/12 cases, including 3 with normozoospermia, 2 with oligozoospermia, 1 with asthenozoospermia, and 2 with teratozoospermia, but not in 4/12 cases, 1 with normozoospermia, 1 with asthenozoospermia, and 2 with oligoasthenozoospermia. PR were undetectable in all cases of negative ER and in 1 case (normozoospermial) of positive ER.
Negative ER and/or PR in spermatozoa from infertile couples might be involved in cases of male infertility.
评估性甾体激素对人类精子作用的临床意义。
从20名男性获取人类精子。采用抗生物素蛋白-生物素-过氧化物酶复合物法进行雌激素受体（ER）和孕激素受体（PR）的免疫组织化学染色。
在所有8对可育夫妇的样本中均观察到ER和PR的阳性免疫组织化学染色。在不育夫妇的样本中，12例中有8例检测到ER，其中3例为正常精子症，2例为少精子症，1例为弱精子症，2例为畸形精子症；但12例中的4例未检测到ER，其中1例为正常精子症，1例为弱精子症，2例为少弱精子症。在所有ER阴性的病例以及1例ER阳性（正常精子症）病例中均未检测到PR。
不育夫妇精子中ER和/或PR阴性可能与男性不育病例有关。
