Felgner P, Lederer I
Tropenmed Parasitol. 1976 Jun;27(2):165-8.
Antigen from axenically and with crithidia monoxenically grown amebae are compared in complement fixation test and indirect hemagglutination test. While similar reactivity was observed in indirect hemagglutination monoxenic antigen yielded extremely higher titers in complement fixation tests. Transient latex agglutinations of severely ill malaria tropica patients proved to be spontaneous in nature. It is recommended that extra latex is added to the test kits to provide for a check of positive sera for spontaneous agglutination. Glutaraldehyde treated and sensitized sheep red cells were stored under different conditions and their reactivity assessed over various periods of time. No loss of reactivity was found to occur under either condition after the respective times of observation, the longest of which lasted 12 months.
在补体结合试验和间接血凝试验中，对无菌培养和单一克氏锥虫培养的变形虫抗原进行了比较。虽然在间接血凝试验中观察到类似的反应性，但单一克氏锥虫培养抗原在补体结合试验中产生的滴度极高。重症热带疟疾患者的短暂乳胶凝集被证明是自发的。建议在试剂盒中添加额外的乳胶，以检查阳性血清是否有自发凝集。对经戊二醛处理并致敏的绵羊红细胞在不同条件下进行储存，并在不同时间段评估其反应性。在各自的观察时间后，在任何一种条件下均未发现反应性丧失，最长观察时间为12个月。
