具有末端G×A错配和3'或5'悬垂碱基的DNA小双链体的热力学和光谱性质。
Thermodynamic and spectral properties of DNA miniduplexes with the terminal G x A mispairs and 3' or 5' dangling bases.
作者信息
Lokhov S G, Pyshnyi D V
机构信息
Institute of Bioorganic Chemistry, Siberian Division, Russian Academy of Science, Novosibirsk.
出版信息
FEBS Lett. 1997 Dec 29;420(2-3):134-8. doi: 10.1016/s0014-5793(97)01502-0.
The tetradeoxyribonucleotide pAGCG in 1 M NaCl forms duplexes with terminal non-canonical pA x G pairs with stability significantly exceeding that for the duplex (pAGCT)2 and lower than that for the duplex (pCGCG)2. The deoxyriboduplex (pAGCG)2 is considerably stabilized by 3'-Y and slightly by 5'-X dangling bases. Therefore, the stability of duplexes with 3' dangling bases decreases in the order (pAGCGY)2 > (pCGCGA)2 > (pAGCTA)2. The sum of the independent stabilizing effect of the of 5'-pG and 3'-A dangling bases on the (pAGCG)2 core duplex is higher than that of the additional terminal pG x A pair in pG-A-/-G-A tandem of the duplex (pGAGCGA)2.
在1 M氯化钠中，四脱氧核糖核苷酸pAGCG与末端非经典pA×G对形成双链体，其稳定性显著超过双链体(pAGCT)2，低于双链体(pCGCG)2。脱氧核糖双链体(pAGCG)2通过3'-Y得到显著稳定，通过5'-X悬垂碱基得到轻微稳定。因此，具有3'悬垂碱基的双链体的稳定性按(pAGCGY)2 > (pCGCGA)2 > (pAGCTA)2的顺序降低。5'-pG和3'-A悬垂碱基对(pAGCG)2核心双链体的独立稳定作用之和高于双链体(pGAGCGA)2的pG-A-/-G-A串联中额外末端pG×A对的稳定作用之和。
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