Motegi F, Nakano K, Kitayama C, Yamamoto M, Mabuchi I
Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Japan.
FEBS Lett. 1997 Dec 29;420(2-3):161-6. doi: 10.1016/s0014-5793(97)01510-x.
We cloned the myo3+ gene of Schizosaccharomyces pombe which encodes a type-II myosin heavy chain. myo3 null cells showed a defect in cytokinesis under certain conditions. Overproduction of Myo3 also showed a defect in cytokinesis. Double mutant analysis indicated that Myo3 genetically interacts with Cdc8 tropomyosin and actin. Myo3 may be implicated in cytokinesis and stabilization of F-actin cables. Moreover, the function of Myo2 can be replaced by overexpressed Myo3. We observed a modest synthetic interaction between Myo2 and Myo3. Thus, Myo2 and Myo3 seem to cooperate in the formation of the F-actin ring in S. pombe.
我们克隆了粟酒裂殖酵母的myo3⁺基因，该基因编码一种II型肌球蛋白重链。在某些条件下，myo3缺失细胞在胞质分裂方面表现出缺陷。Myo3的过量表达也显示出胞质分裂缺陷。双突变分析表明，Myo3在遗传上与Cdc8原肌球蛋白和肌动蛋白相互作用。Myo3可能参与胞质分裂和F-肌动蛋白电缆的稳定。此外，Myo2的功能可以被过表达的Myo3取代。我们观察到Myo2和Myo3之间存在适度的合成相互作用。因此，Myo2和Myo3似乎在粟酒裂殖酵母中F-肌动蛋白环的形成中协同作用。
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