Battelli M G, Buonamici L, Virgili M, Abbondanza A, Contestabile A
Department of Experimental Pathology, University of Bologna, Italy.
Neurochem Int. 1998 Jan;32(1):17-21. doi: 10.1016/s0197-0186(97)00052-1.
Xanthine dehydrogenase and oxidase activities increased by 87% in rat brain slices after 30 min in vitro ischaemia. A further 41% increase was induced by 30 min simulated reperfusion of ischaemic slices. No conversion from the dehydrogenase to the oxidase activity was observed. The increment of enzyme activity was not due to neosynthesis of the enzyme, since it was not affected by the addition of cycloheximide during the ischaemic incubation. The increased oxygen-dependent form of the enzyme could aggravate the ischaemic brain injury by free radicals production, in particular after reperfusion.
在体外缺血30分钟后，大鼠脑片中的黄嘌呤脱氢酶和氧化酶活性增加了87%。对缺血脑片进行30分钟的模拟再灌注后，酶活性又进一步增加了41%。未观察到从脱氢酶活性向氧化酶活性的转化。酶活性的增加并非由于酶的重新合成，因为在缺血孵育期间添加环己酰亚胺对其没有影响。这种酶的氧依赖性形式增加，可能通过产生自由基加重缺血性脑损伤，特别是在再灌注后。
