Mencarelli C, Cotelli F
Department of Evolutionary Biology, University of Siena, Italy.
Zygote. 1997 Aug;5(3):207-12. doi: 10.1017/s0967199400003634.
We have used monoclonal antibodies specific for different sets of human cytokeratins and the anti-IFA (Intermediate Filament Antigen) antibody to investigate the expression of intermediate filament proteins in the mature oocyte of the teleost Cyprinus carpio. Several polypeptides have been identified, showing molecular weights ranging from 43 to 65 kDa. Two-dimensional analysis of the immunoreactive species revealed the presence of at least six major protein spots and a series of minor components, grouped in quite a narrow pI range from 5.52 to 6.28. The general complexity of the carp oocyte cytokeratin-related cytoskeleton appears to be higher than those described for oocytes of other vertebrate species.
我们使用了针对不同人细胞角蛋白组的单克隆抗体以及抗中间丝抗原（IFA）抗体，来研究硬骨鱼鲤成熟卵母细胞中中间丝蛋白的表达。已鉴定出几种多肽，其分子量范围为43至65 kDa。对免疫反应性物质的二维分析显示，存在至少六个主要蛋白斑点以及一系列次要成分，它们聚集在相当窄的pH值范围5.52至6.28内。鲤卵母细胞角蛋白相关细胞骨架的总体复杂性似乎高于其他脊椎动物物种卵母细胞所描述的复杂性。
