Le Gall G, Arnauld C, Boilletot E, Morisse J P, Rasschaert D
Unité de Virologie, Immunologie, Parasitologie Aviaires et Cunicoles, Centre National d'Etudes Vétérinaires et Alimentaires, Zoopôle, Ploufragan, France.
J Gen Virol. 1998 Jan;79 ( Pt 1):11-6. doi: 10.1099/0022-1317-79-1-11.
In order to evaluate genetic variation between rabbit haemorrhagic disease virus (RHDV) isolates and to derive phylogenetic relationships, 56 virus isolates collected from various parts of France over a 7 year period (1988 to 1995) were examined. Analyses were carried out by direct nucleotide sequencing of PCR fragments of three genomic regions encoding the capsid protein (VP60) (regions A and B) and a non-structural protein (region C). Multiple sequence alignments revealed maximum nucleotide divergence of 7.6, 9.4 and 8.7% for regions A, B and C, respectively, indicating a high level of conservation between isolates. Irrespective of the genomic region analysed, phylogenetic analyses carried out using various methods allowed the identification of three genogroups; distribution of isolates within these genogroups appears to be more related to the year of their collection than to their geographical origin. The possible evolution of RHDV is discussed.
为了评估兔出血症病毒（RHDV）分离株之间的遗传变异并推导系统发育关系，对在7年期间（1988年至1995年）从法国各地收集的56株病毒分离株进行了检测。通过对编码衣壳蛋白（VP60）的三个基因组区域（区域A和B）和一个非结构蛋白（区域C）的PCR片段进行直接核苷酸测序来进行分析。多重序列比对显示，区域A、B和C的最大核苷酸差异分别为7.6%、9.4%和8.7%，表明分离株之间具有高度的保守性。无论分析的基因组区域如何，使用各种方法进行的系统发育分析都能鉴定出三个基因群；这些基因群内分离株的分布似乎与其收集年份的关系比与其地理来源的关系更大。文中讨论了RHDV可能的进化情况。
