Carvalho Carina Luísa, Silva Sara, Gouveia Paz, Costa Margarida, Duarte Elsa Leclerc, Henriques Ana Margarida, Barros Sílvia Santos, Luís Tiago, Ramos Fernanda, Fagulha Teresa, Fevereiro Miguel, Duarte Margarida Dias
Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Universidade de Évora, Núcleo da Mitra, 7000, Évora, Portugal.
Instituto de Investigação e Formação Avançada (IIFA), Universidade de Évora, Núcleo da Mitra, 7000, Évora, Portugal.
Virus Genes. 2017 Dec;53(6):922-926. doi: 10.1007/s11262-017-1483-6. Epub 2017 Jun 21.
We report the detection of rabbit haemorrhagic disease virus 2 (RHDV2) in the Madeira archipelago, Portugal. Viral circulation was confirmed by RT-qPCR and vp60 sequencing. Epidemiological data revealed the outbreak initiated in October 2016 in Porto Santo affecting wild and domestic rabbits. It was then detected three months later on the island of Madeira. Five haplotypes were identified and a genetic overall similarity of 99.54 to 99.89% was observed between the two viral populations. Unique single nucleotide polymorphisms were recognised in the Madeira archipelago strains, two of which resulting in amino acid substitutions at positions 480 and 570 in the VP60 protein. Phylogenetic investigation by Maximum Likelihood showed all the vp60 sequences from the Madeira archipelago group together with high bootstraps. The analysis also showed that the Madeira archipelago strains are closely related to the strains detected in the south of mainland Portugal in 2016, suggesting a possible introduction from the mainland. The epidemiological data and high genetic similarity indicate a common source for the Porto Santo and Madeira RHDV2 outbreaks. Human activity related to hunting was most probably at the origin of the Madeira outbreak.
我们报告了在葡萄牙马德拉群岛检测到兔出血症病毒2型（RHDV2）。通过逆转录定量聚合酶链反应（RT-qPCR）和vp60基因测序确认了病毒的传播。流行病学数据显示，疫情于2016年10月在圣港岛爆发，影响野生和家养兔子，三个月后在马德拉岛被检测到。鉴定出五个单倍型，两个病毒群体之间的遗传总体相似性为99.54%至99.89%。在马德拉群岛的毒株中识别出独特的单核苷酸多态性，其中两个导致VP60蛋白第480和570位氨基酸发生替换。通过最大似然法进行的系统发育研究表明，来自马德拉群岛的所有vp60序列聚集在一起，自展值很高。分析还表明，马德拉群岛的毒株与2016年在葡萄牙大陆南部检测到的毒株密切相关，这表明可能是从大陆传入。流行病学数据和高度的遗传相似性表明，圣港岛和马德拉岛的RHDV2疫情有共同的源头。与狩猎相关的人类活动很可能是马德拉岛疫情的起源。
