Tsafriri A, Lieberman M E, Ahrén K, Lindner H R
Acta Endocrinol (Copenh). 1976 Feb;81(2):362-6. doi: 10.1530/acta.0.0810362.
Luteinizing hormone (NIH-LH-S18; 5 mug/ml) stimulated aerobic glycolysis in cultured Graafian follicles explanted from pro-oestrous rats before the preovulatory gonadotrophin surge: lactate accumulation in the medium was 70% above control levels during 6 h incubations. Iodoacetate (2.5 x 10(-5) M) prevented this effect, without impairing the ability of LH to induce resumption of oocytic meiosis. Enrichment of the medium with pyruvate (3.3 x 10(-4) M) or lactate (2.5 x 10(-2) M) did not in itself cause ovum maturation. The results do not support the hypothesis that termination of meiotic arrest by LH is due to stimulation of glycolytic activity in the follicle cells, resulting in increased availability of an energy source readily urilizable by the oocyte.
促黄体生成素（NIH-LH-S18；5微克/毫升）在排卵前促性腺激素激增之前，刺激了从动情前期大鼠取出的培养格拉夫卵泡中的有氧糖酵解：在6小时的孵育过程中，培养基中的乳酸积累比对照水平高70%。碘乙酸（2.5×10⁻⁵ M）可防止这种作用，而不损害促黄体生成素诱导卵母细胞减数分裂恢复的能力。用丙酮酸（3.3×10⁻⁴ M）或乳酸（2.5×10⁻² M）富集培养基本身不会导致卵子成熟。这些结果不支持以下假设，即促黄体生成素终止减数分裂阻滞是由于刺激卵泡细胞中的糖酵解活性，从而导致卵母细胞易于利用的能量来源可用性增加。
