Killenberg P G, Dukes D F
J Lipid Res. 1976 Sep;17(5):451-55.
Synthesis of the coenzyme A derivatives of bile acids by the mixed anyhydride method results in a product that is contaminated by unreacted CoASH and bile acid. These compounds can be purified by Sephadex LH-20 chromatography. In each case, the purified product is free of starting materials and exhibits an equimolar ratio of bile acid, coenzyme A, and thioester bond. Millimolar extinction coefficients were calculated for these compounds as A259 nm, 15.03 +/- 0.58; A232 nm, 7.60 +/- 0.17; and A232 nm for the thioester bond, 4.12 +/- 0.17. These CoA derivatives were hydrolyzed in strongly alkaline solution, but were stable at physiologic temperature and pH. Utilization of these compounds in the enzymic preparation of taurine conjugates of bile acids indicated 94% activity. These purified CoA derivatives may be useful in studying the enzymic conjugation of bile acids.
通过混合酸酐法合成胆汁酸的辅酶A衍生物会得到一种被未反应的辅酶A和胆汁酸污染的产物。这些化合物可通过葡聚糖凝胶LH - 20柱色谱法进行纯化。在每种情况下，纯化后的产物不含起始原料，且胆汁酸、辅酶A和硫酯键呈现等摩尔比。计算出这些化合物的毫摩尔消光系数为：在259nm处，A为15.03±0.58；在232nm处，A为7.60±0.17；硫酯键在232nm处，A为4.12±0.17。这些辅酶A衍生物在强碱性溶液中会水解，但在生理温度和pH条件下稳定。在胆汁酸的牛磺酸共轭物的酶促制备中使用这些化合物显示出94%的活性。这些纯化的辅酶A衍生物可能有助于研究胆汁酸的酶促共轭作用。
