Farombi E O, Nwankwo J O, Emerole G O
Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria.
Food Chem Toxicol. 1997 Oct-Nov;35(10-11):975-9. doi: 10.1016/s0278-6915(97)87266-3.
The possible modulatory effect of browned yam flour, a local dietary staple in south western Nigeria, on the toxicity of 7,12-dimethylbenzanthracene (DMBA), 3-methylcholanthrene (3-MC), carbon tetrachloride (CCl4) and bromobenzene (BrB) in rats was investigated. Feeding rats with 25% browned yam flour 2 wk before treatment with 65 mg/kg DMBA (single dose) and 5 mg/kg 3-MC and continued for 3 wk significantly decreased the reduction in final body weight or weight gain and organ weights caused by the two compounds. Similarly, the diet decreased the reduction in body weight or weight gain and the increase in relative liver weight mediated by oral treatment with 0.5 ml CCl4/kg and 2.5 mmol BrB/kg body weight. Incorporation of 25% browned yam flour into rat diet significantly reduced the DMBA-mediated decrease in haemoglobin content, packed cell volume, red blood cell count and white blood cell count by 7, 5, 20 and 10%, respectively; while the diet reduced the 3-MC-mediated decrease in these parameters by 15, 28, 9 and 17%, respectively. The same diet elicited 23, 45, 13 and 33% decreases in CCl4 mediated reduction in these parameters and 23, 18, 16 and 29% in the case of BrB. Browned yam flour diet caused 10, 14 (P < 0.001) and 4% (P < 0.05) reductions in the DMBA-mediated increase in serum aspartate aminotransferase, alanine aminotransferase and serum alkaline phosphatase, respectively; and 32, 31 (P < 0.05) and 13% (P < 0.001) in the case of the 3-MC-mediated increase. Also, the diet reduced CCl4-mediated increase in the activities of these by 40, 34 and 31%, respectively and by 23, 30 and 29% following BrB treatment. These results suggest that browned yam flour diet could possibly be a modulator of chemically induced toxicity.
研究了尼日利亚西南部当地主食——褐变山药粉对大鼠体内7,12 - 二甲基苯并蒽（DMBA）、3 - 甲基胆蒽（3 - MC）、四氯化碳（CCl4）和溴苯（BrB）毒性的潜在调节作用。在用65 mg/kg DMBA（单剂量）和5 mg/kg 3 - MC处理大鼠前2周，给大鼠喂食25%的褐变山药粉，并持续3周，这显著减轻了这两种化合物导致的最终体重或体重增加以及器官重量的降低。同样，该饮食减少了口服0.5 ml CCl4/kg和2.5 mmol BrB/kg体重所介导的体重或体重增加的减少以及相对肝脏重量的增加。将25%的褐变山药粉加入大鼠饮食中，显著降低了DMBA介导的血红蛋白含量、血细胞比容、红细胞计数和白细胞计数的降低，降幅分别为7%、5%、20%和10%；而该饮食使3 - MC介导的这些参数的降低分别减少了15%、28%、9%和17%。同样的饮食使CCl4介导的这些参数的降低分别减少了23%、45%、13%和33%，对于BrB则分别减少了23%、18%、16%和29%。褐变山药粉饮食使DMBA介导的血清天冬氨酸转氨酶、丙氨酸转氨酶和血清碱性磷酸酶的增加分别降低了10%、14%（P < 0.001）和4%（P < 0.05）；对于3 - MC介导的增加，分别降低了32%、31%（P < 0.05）和13%（P < 0.001）。此外，该饮食使CCl4介导的这些酶活性的增加分别降低了40%、34%和31%，BrB处理后分别降低了23%、30%和29%。这些结果表明，褐变山药粉饮食可能是化学诱导毒性的调节剂。
