编码支链氨基酸转运蛋白的金黄色葡萄球菌基因的克隆与核苷酸测序
Cloning and nucleotide sequencing of a Staphylococcus aureus gene encoding a branched-chain-amino-acid transporter.
作者信息
Vijaranakul U, Xiong A, Lockwood K, Jayaswal R K
机构信息
Department of Biological Sciences, Illinois State University, Normal 61790-4120, USA.
出版信息
Appl Environ Microbiol. 1998 Feb;64(2):763-7. doi: 10.1128/AEM.64.2.763-767.1998.
Abstract
We recently characterized a transposon-induced NaCl-sensitive mutant of Staphylococcus aureus (U. Vijaranakul, M. J. Nadakavukaren, D. O. Bayles, B. J. Wilkinson, and R. K. Jayaswal, Appl. Environ. Microbiol. 63:1889-1897, 1997). To further characterize this mutant, we determined the nucleotide sequence at the insertion site of the transposon on the S. aureus chromosome. Nucleotide sequencing revealed a 1,326-bp open reading frame (ORF442) encoding a hydrophobic 442-amino-acid polypeptide with a calculated molecular mass of 49,058 Da. The hydrophilicity profile of the gene product revealed the existence of 12 hydrophobic domains predicted to form membrane-associated alpha-helices. Comparison of the amino acid sequence of ORF442 with amino acid sequences in the GenBank database showed extensive homology with the branched-chain-amino-acid transport genes of gram-positive and gram-negative bacteria. This is the first brnQ gene in staphylococci to be described.
摘要
我们最近鉴定了一株由转座子诱导的金黄色葡萄球菌NaCl敏感突变体（U. Vijaranakul、M. J. Nadakavukaren、D. O. Bayles、B. J. Wilkinson和R. K. Jayaswal，《应用与环境微生物学》63:1889 - 1897，1997年）。为了进一步鉴定该突变体，我们测定了转座子在金黄色葡萄球菌染色体上插入位点的核苷酸序列。核苷酸测序揭示了一个1326 bp的开放阅读框（ORF442），其编码一个疏水性的442个氨基酸的多肽，计算分子量为49058 Da。该基因产物的亲水性图谱显示存在12个预测形成膜相关α螺旋的疏水区。将ORF442的氨基酸序列与GenBank数据库中的氨基酸序列进行比较，结果表明它与革兰氏阳性和革兰氏阴性细菌的支链氨基酸转运基因具有广泛的同源性。这是首次在葡萄球菌中描述的brnQ基因。
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