Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tennodai 1-1-1, Tsukuba 305-8575, Japan.
BMC Microbiol. 2011 Jan 18;11:13. doi: 10.1186/1471-2180-11-13.
The ability of staphylococci to grow in a wide range of salt concentrations is well documented. In this study, we aimed to clarify the role of cardiolipin (CL) in the adaptation of Staphylococcus aureus to high salinity.
Using an improved extraction method, the analysis of phospholipid composition suggested that CL levels increased slightly toward stationary phase, but that this was not induced by high salinity. Deletion of the two CL synthase genes, SA1155 (cls1) and SA1891 (cls2), abolished CL synthesis. The cls2 gene encoded the dominant CL synthase. In a cls2 deletion mutant, Cls1 functioned under stress conditions, including high salinity. Using these mutants, CL was shown to be unnecessary for growth in either basal or high-salt conditions, but it was critical for prolonged survival in high-salt conditions and for generation of the L-form.
CL is not essential for S. aureus growth under conditions of high salinity, but is necessary for survival under prolonged high-salt stress and for the generation of L-form variants.
葡萄球菌能够在广泛的盐浓度范围内生长，这一现象已得到充分证实。在本研究中，我们旨在阐明心磷脂（CL）在金黄色葡萄球菌适应高盐环境中的作用。
使用改进的提取方法，对磷脂组成的分析表明，CL 水平在静止期略有上升，但这不是由高盐诱导的。删除两个 CL 合酶基因 SA1155（cls1）和 SA1891（cls2），会导致 CL 合成被废除。cls2 基因编码主要的 CL 合酶。在 cls2 缺失突变体中，Cls1 在包括高盐在内的应激条件下发挥作用。使用这些突变体，表明 CL 对于基础盐度或高盐度条件下的生长不是必需的，但对于在高盐度环境中长时间存活和 L 型变体的产生至关重要。
CL 对于金黄色葡萄球菌在高盐条件下的生长不是必需的，但对于在长时间高盐应激下的存活和 L 型变体的产生是必需的。
