Atrial natriuretic peptide mediated alterations in catecholamine and indoleamine turnover in the nucleus of the solitary tract of rats.

The effect of locally perfused atrial natriuretic peptide (ANP) into the nucleus of the solitary tract (NST) was studied by monitoring the changes in the extracellular neurotransmitters and the cardiovascular functions in urethane anesthetized Sprague-Dawley rats. Perfusion of ANP was accomplished by using in vivo microdialysis probe unilaterally and stereotaxically implanted into the NST area. ANP at a concentration of 4 x 10(-12) M was perfused at a constant flow rate of 1.5 microl/min. over a period of one hour and the dialysate was assayed for both catecholamines and indoleamine. Perfusion of ANP into the NST led to significant reduction in both blood pressure and heart rate. Serotonin turnover was unaffected. Both norepinephrine (NE) and methoxyhydroxyphenylglycol (MHPG) levels increased, while the dihydroxyphenylacetic acid (DOPAC) level decreased, indicating the possible interaction between ANP and the catecholaminergic system of the NST in regulating the arterial blood pressure.