Rowntree S, Kolb B
Department of Psychology, University of Lethbridge, AB, Canada.
Eur J Neurosci. 1997 Nov;9(11):2432-41. doi: 10.1111/j.1460-9568.1997.tb01660.x.
The endogenous expression of basic fibroblast growth factor (bFGF) was blocked by neutralizing antibodies following unilateral suction lesions of the motor cortex. Rats with control treatment (saline, goat IgG) after motor cortex lesions showed slow recovery of forelimb manipulatory abilities. Rats with blockade of bFGF expression showed little recovery. Anatomically, the control-treated lesioned rats showed an acute increase in bFGF and glial fibrillary acidic protein (GFAP) reactivity, and chronically they had normal dendritic arborization and spine density in layer V pyramidal cells in the remaining motor cortex. In contrast, rats treated with antibodies to bFGF showed little bFGF reactivity, normal GFAP reactivity, and atrophy of dendritic arbor and decreased spine density in layer V pyramidal cells. These results demonstrate the importance of endogenous bFGF release in processes related to functional recovery after cortical injury.
在运动皮层单侧抽吸损伤后，通过中和抗体阻断碱性成纤维细胞生长因子（bFGF）的内源性表达。运动皮层损伤后接受对照处理（生理盐水、山羊IgG）的大鼠，其前肢操作能力恢复缓慢。bFGF表达被阻断的大鼠几乎没有恢复。在解剖学上，接受对照处理的损伤大鼠bFGF和胶质纤维酸性蛋白（GFAP）反应性急性增加，长期来看，它们在剩余运动皮层的V层锥体细胞中具有正常的树突分支和棘密度。相比之下，用抗bFGF抗体处理的大鼠几乎没有bFGF反应性，GFAP反应性正常，树突分支萎缩，V层锥体细胞的棘密度降低。这些结果证明了内源性bFGF释放在皮层损伤后功能恢复相关过程中的重要性。
