Thi A D, Evrard C, Rouget P
Laboratorie Biologie Moléculaire et Différenciation, Unité de Génétique Oncologique, CNRS-URA 1967, Insitut Gustave Roussy, Villejuif, France.
J Exp Biol. 1998 Mar;201(Pt 6):851-60. doi: 10.1242/jeb.201.6.851.
Permanent Schwann cells lines have been established in culture after stable transfection of newborn rat Schwann cells with the pJC-SVLTtsA vector, expressing a thermosensitive oncogene driven by the early promoter-enhancer region of the gliotropic GS/B variant of the papovavirus JC. The proliferation and differentiation of two clonal cell lines have been studied. The cells of these lines display the morphology of primary Schwann cells and express Schwann cell differentiation markers such as the S-100 protein, laminin, the low-affinity receptor to nerve growth factor and the glial fibrillary acidic protein. One of the lines is able to differentiate further. Indeed, in the presence of dorsal root ganglion neurones, the cells synthesize the myelin Po protein and are capable of some myelination, although to a lesser extent than secondary Schwann cells.
用pJC - SVLTtsA载体稳定转染新生大鼠雪旺细胞后，已在培养中建立了永久性雪旺细胞系。该载体表达一种由乳头多瘤空泡病毒JC的嗜神经GS/B变体的早期启动子 - 增强子区域驱动的温度敏感致癌基因。研究了两个克隆细胞系的增殖和分化。这些细胞系的细胞呈现原代雪旺细胞的形态，并表达雪旺细胞分化标志物，如S - 100蛋白、层粘连蛋白、神经生长因子低亲和力受体和胶质纤维酸性蛋白。其中一个细胞系能够进一步分化。实际上，在背根神经节神经元存在的情况下，这些细胞合成髓磷脂Po蛋白，并且能够进行一定程度的髓鞘形成，尽管程度比次级雪旺细胞小。
