Cayanis E, Russo J J, Kalachikov S, Ye X, Park S H, Sunjevaric I, Bonaldo M F, Lawton L, Venkatraj V S, Schon E, Soares M B, Rothstein R, Warburton D, Edelman I S, Zhang P, Efstratiadis A, Fischer S G
Columbia Genome Center, Columbia University, New York, New York 10032, USA.
Genomics. 1998 Jan 1;47(1):26-43. doi: 10.1006/geno.1997.5087.
We have assembled a high-resolution physical map of human chromosome 13 DNA (approximately 114 Mb) from hybridization, PCR, and FISH mapping data using a specifically designed set of computer programs. Although the mapping of 13p is limited, 13q (approximately 98 Mb) is covered by an almost continuous contig of 736 YACs aligned to 597 contigs of cosmids. Of a total of 10,789 cosmids initially selected from a chromosome 13-specific cosmid library (16,896 colonies) using inter-Alu PCR probes from the YACs and probes for markers mapped to chromosome 13, 511 were assembled in contigs that were established from cross-hybridization relationships between the cosmids. The 13q YAC-cosmid map was annotated with 655 sequence tagged sites (STSs) with an average spacing of 1 STS per 150 kb. This set of STSs, each identified by a D number and cytogenetic location, includes database markers (198), expressed sequence tags (93), and STSs generated by sequencing of the ends of cosmid inserts (364). Additional annotation has been provided by positioning 197 cosmids mapped by FISH on 13q. The final (comprehensive) map, a list of STS primers, and raw data used in map assembly are available at our Web site (genome1.ccc.columbia.edu/ approximately genome/) and can serve as a resource to facilitate accurate localization of additional markers, provide substrates for sequencing, and assist in the discovery of chromosome 13 genes associated with hereditary diseases.
我们利用一套专门设计的计算机程序，通过杂交、聚合酶链反应（PCR）和荧光原位杂交（FISH）图谱数据，构建了人类13号染色体DNA的高分辨率物理图谱（约114兆碱基）。尽管13p的图谱绘制有限，但13q（约98兆碱基）由736个酵母人工染色体（YAC）的几乎连续的重叠群覆盖，这些YAC与597个粘粒重叠群对齐。从一个13号染色体特异性粘粒文库（16,896个菌落）中最初使用来自YAC的Alu间PCR探针和映射到13号染色体的标记探针选择的总共10,789个粘粒中，511个被组装成基于粘粒之间交叉杂交关系建立的重叠群。13q的YAC - 粘粒图谱用655个序列标签位点（STS）进行注释，平均间距为每150 kb一个STS。这组STS，每个都由一个D编号和细胞遗传学位置标识，包括数据库标记（198个）、表达序列标签（93个）以及通过对粘粒插入片段末端测序产生的STS（364个）。通过将197个通过FISH映射到13q的粘粒定位，提供了额外的注释。最终（综合）图谱、STS引物列表以及图谱组装中使用的原始数据可在我们的网站（genome1.ccc.columbia.edu/ approximately genome/）获取，可作为促进额外标记精确定位、提供测序底物以及协助发现与遗传性疾病相关的13号染色体基因的资源。
