Hauser P M, Blanc D S, Bille J, Telenti A, Francioli P
Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.
APMIS Suppl. 1997;77:7-10. doi: 10.1111/j.1600-0463.1997.tb05373.x.
We are developing a typing method for Pneumocystis carinii sp.f. hominis consisting in the PCR amplification of four variable regions of the genome from bronchoalveolar lavages, followed by the detection of their variation by the single-strand conformation polymorphism technique (SSCP). Most samples of each region from eleven unrelated patients showed two SSCP bands. Six patients were probably infected by a single strain since they showed a single sequence morph of each region. The combinations of the four sequence morphs of these patients were all different so that six different strains were distinguished. Other samples generated three or four SSCP bands which were found to correspond to the presence of two sequence morphs, possibly due to co-infections. Five patients could have been co-infected since they showed two sequence morphs of at least one of the regions. The combinations of sequence morphs of the possible co-infecting strains were different from all other combinations, except that three possible strains in two patients might have been present in other patients. Two BALs from the same patient, which were collected at an interval of 2.5 weeks, gave the same pattern for each of the four regions suggesting that these regions of the genome are stable. These results suggest a great diversity of P. carinii sp.f. hominis strains. Thus, the method should be suitable for epidemiological studies.
我们正在开发一种针对人肺孢子菌的分型方法，该方法包括从支气管肺泡灌洗物中对基因组的四个可变区域进行PCR扩增，随后通过单链构象多态性技术（SSCP）检测其变异情况。来自11名不相关患者的每个区域的大多数样本显示出两条SSCP带。6名患者可能感染了单一菌株，因为他们每个区域都显示出单一的序列形态。这些患者的四种序列形态组合均不相同，因此区分出了六种不同的菌株。其他样本产生了三条或四条SSCP带，发现这与两种序列形态的存在相对应，可能是由于合并感染。5名患者可能发生了合并感染，因为他们至少在一个区域显示出两种序列形态。可能的合并感染菌株的序列形态组合与所有其他组合不同，不过两名患者中三种可能的菌株可能也存在于其他患者中。同一患者间隔2.5周采集的两份支气管肺泡灌洗物，四个区域中的每个区域都呈现相同的模式，这表明基因组的这些区域是稳定的。这些结果表明人肺孢子菌菌株具有很大的多样性。因此，该方法应适用于流行病学研究。
