Harada H, Mitsuyasu T, Seta Y, Maruoka Y, Toyoshima K, Yasumoto S
Second Department of Oral Anatomy and Cell Biology, Kyushu Dental College, Kitakyushu, Japan.
J Oral Pathol Med. 1998 Jan;27(1):11-7. doi: 10.1111/j.1600-0714.1998.tb02084.x.
The bcl-2 proto-oncogene is a known inhibitor of apoptosis; in normal human stratified squamous epithelium, its expression is restricted to the basal cell layer. To investigate the functional role of bcl-2 protein in the process of differentiation of oral keratinocytes, bcl-2 expression vector was transfected into SCC-25 cells, which normally undergo squamous cell differentiation in vitro while expressing specific differentiation markers, e.g., keratin 10/11 and involucrin. In bcl-2 transfected SCC-25 cells, the expression of these differentiation markers was markedly suppressed. The bcl-2 proto-oncogene may play a critical role in opposing the commitment to terminal differentiation and apoptosis of oral keratinocytes.
bcl-2原癌基因是一种已知的细胞凋亡抑制剂；在正常人类复层鳞状上皮中，其表达仅限于基底细胞层。为了研究bcl-2蛋白在口腔角质形成细胞分化过程中的功能作用，将bcl-2表达载体转染到SCC-25细胞中，该细胞在体外通常会经历鳞状细胞分化，同时表达特定的分化标志物，如角蛋白10/11和内披蛋白。在转染了bcl-2的SCC-25细胞中，这些分化标志物的表达被明显抑制。bcl-2原癌基因可能在对抗口腔角质形成细胞的终末分化和凋亡过程中起关键作用。
