Namgoong S Y, Kim K, Saxena P, Yang J Y, Jayaram M, Giedroc D P, Harshey R M
Department of Microbiology, University of Texas at Austin 78712, USA.
J Mol Biol. 1998 Jan 16;275(2):221-32. doi: 10.1006/jmbi.1997.1466.
This study examines the contribution of domain II beta of bacteriophage Mu transposase (A protein), a subdomain of the central catalytic domain II, to the transposition reaction. The properties of several point mutations implicate a role for this domain in facilitating metal-assisted assembly of the synaptic complex, as well as in intramolecular DNA strand transfer. Point mutations as well as deletions in domain II beta can be complemented by those in domain II alpha but not those in domain III alpha. Thus, residues within subdomains II alpha and II beta belong to different catalytic complementation groups.
本研究考察了噬菌体Mu转座酶（A蛋白）中心催化结构域II的一个亚结构域——结构域IIβ对转座反应的作用。几个点突变的特性表明，该结构域在促进金属辅助的突触复合体组装以及分子内DNA链转移中发挥作用。结构域IIβ中的点突变和缺失可被结构域IIα中的突变互补，但不能被结构域IIIα中的突变互补。因此，亚结构域IIα和IIβ内的残基属于不同的催化互补组。
