Jørgensen H, Zhao X Q
Danish Institute of Animal Science, Department of Nutrition, Research Center Foulum, Tjele, Denmark.
Z Ernahrungswiss. 1997 Dec;36(4):278-84. doi: 10.1007/BF01617798.
Seventy two Wistar rats were used in two repeat studies to investigate the effect of environmental temperature (18 degrees C or 28 degrees C) and increasing levels of dietary fibre (low, 68 g/kg DM; medium 110 g/kg DM; high, 157 g/kg DM) or protein (low, 91 g/kg DM; medium, 171 g/kg DM; high, 262 g/kg DM) on digestive tract, visceral organ size, energy metabolism, and respiration attributable to Na+,K(+)-ATPase activity in duodenal mucosa and liver. Total and ouabain-sensitive (a measure of Na+,K(+)-ATPase activity) O2 consumption in vitro of tissues were measured polarographically using a Clark-style YSI biological O2 monitor. Whole body heat production (in vivo) was measured using open-circuit respiration chambers. The weight of the visceral organs was higher in rats housed at 18 degrees C than at 28 degrees C. The empty weight of the small intestine, caecum, and colon increased as the level of dietary fibre increased (P 0.05). Heat production as a proportion of metabolizable energy was higher (P < 0.05) at 18 degrees C than at 28 degrees C in the first experiment but this difference was significant in the second experiment. Rats fed the low protein diet had significantly higher (P > 0.05) heat production than those fed medium or high protein diets. Compared to 28 degrees C, environmental temperature of 18 degrees C caused an increased total and ouabain-sensitive O2 consumption in duodenal mucosa. There was no significant effect of environmental temperature on total and ouabain-sensitive O2 consumption in the liver. However, ouabain-sensitive O2 consumption in liver was significantly higher (P 0.05) when rats were fed a low protein diet compared to the medium or high protein diet. Total and ouabain-sensitive O2 consumption increased in duodenal mucosa of rats fed low level of dietary fibre compared to the medium or high dietary fibre diets. The in vitro results corresponded with the whole animal energy expenditure and O2 consumption in vivo.
在两项重复研究中使用了72只Wistar大鼠，以研究环境温度（18摄氏度或28摄氏度）以及膳食纤维水平增加（低，68克/千克干物质；中，110克/千克干物质；高，157克/千克干物质）或蛋白质水平增加（低，91克/千克干物质；中，171克/千克干物质；高，262克/千克干物质）对消化道、内脏器官大小、能量代谢以及十二指肠黏膜和肝脏中Na +,K(+)-ATP酶活性所致呼吸的影响。使用Clark型YSI生物氧监测仪通过极谱法测量组织的总耗氧量和哇巴因敏感耗氧量（Na +,K(+)-ATP酶活性的一种度量）。使用开路呼吸室测量全身产热（体内）。饲养在18摄氏度环境中的大鼠内脏器官重量高于28摄氏度环境中的大鼠。随着膳食纤维水平的增加，小肠、盲肠和结肠的空重增加（P < 0.05）。在第一个实验中，18摄氏度时产热占可代谢能量的比例高于28摄氏度时（P < 0.05），但在第二个实验中这种差异显著。喂食低蛋白饮食的大鼠产热显著高于喂食中蛋白或高蛋白饮食的大鼠（P > 0.05）。与28摄氏度相比，18摄氏度的环境温度导致十二指肠黏膜中总耗氧量和哇巴因敏感耗氧量增加。环境温度对肝脏中的总耗氧量和哇巴因敏感耗氧量没有显著影响。然而，与中蛋白或高蛋白饮食相比，喂食低蛋白饮食的大鼠肝脏中哇巴因敏感耗氧量显著更高（P < 0.05）。与中膳食纤维或高膳食纤维饮食相比，喂食低水平膳食纤维的大鼠十二指肠黏膜中总耗氧量和哇巴因敏感耗氧量增加。体外实验结果与动物整体能量消耗和体内耗氧量相符。
