McBride B W, Milligan L P
Br J Nutr. 1985 May;53(3):605-14. doi: 10.1079/bjn19850070.
Oxygen consumption and Na+,K+-ATPase(EC 3.6.1.3)-dependent (ouabain-sensitive) and -independent respiration were measured for duodenal mucosa biopsies from 10-month-old sheep given two levels of digestible energy (DE) intake (7.6-7.7 and 14.8 MJ lucerne (Medicago sativa) pellets/d) and following 48 h of starvation. The mucosal biopsies were determined to be structurally intact and free of adherent bacteria on histological and scanning-electron-microscope examinations. The use of D-glucose as a substrate during incubations did not elevate (P greater than 0.05) the respiration indices of the biopsies over those measured during acetate incubations. Glucose uptake did not (P greater than 0.05) influence the Na+,K+-ATPase-dependent respiration of the mucosal biopsies. Na+,K+-ATPase-dependent respiration accounted for 50% of the total O2 consumption of the mucosal biopsies of sheep given the lower level of DE. Total O2 consumption of the duodenal mucosa was not (P greater than 0.05) increased when sheep were given the higher level of DE but Na+,K+-ATPase-dependent respiration of the mucosa was elevated (P less than 0.01) by 37% during this period. When sheep were starved for 48 h, total O2 consumption of the mucosal biopsies was not (P greater than 0.05) affected, however, Na+,K+-ATPase-dependent respiration of the biopsies dropped (P less than 0.01) by 45%. Na+,K+-ATPase-dependent respiration accounted for 61.3% of the O2 uptakes of mucosa from the sheep given the higher level of DE and 28.3% of the O2 uptake of mucosa from fasted sheep.
对10月龄绵羊十二指肠黏膜活检样本进行了氧消耗以及钠钾ATP酶（EC 3.6.1.3）依赖性（哇巴因敏感）呼吸和非依赖性呼吸的测定。这些绵羊分别给予两种可消化能量（DE）摄入量水平（7.6 - 7.7和14.8 MJ紫花苜蓿（紫花苜蓿）颗粒/天），并在饥饿48小时后进行测定。经组织学和扫描电子显微镜检查，确定黏膜活检样本结构完整且无黏附细菌。在孵育过程中使用D - 葡萄糖作为底物，并未使活检样本的呼吸指数高于在醋酸盐孵育期间测得的指数（P大于0.05）。葡萄糖摄取对黏膜活检样本的钠钾ATP酶依赖性呼吸没有影响（P大于0.05）。给予较低DE水平的绵羊，其黏膜活检样本中钠钾ATP酶依赖性呼吸占总氧消耗的50%。当给予绵羊较高DE水平时，十二指肠黏膜的总氧消耗没有增加（P大于0.05），但在此期间黏膜的钠钾ATP酶依赖性呼吸升高了37%（P小于0.01）。当绵羊饥饿48小时时，黏膜活检样本的总氧消耗未受影响（P大于0.05），然而，活检样本的钠钾ATP酶依赖性呼吸下降了45%（P小于0.01）。给予较高DE水平的绵羊，其黏膜氧摄取中钠钾ATP酶依赖性呼吸占61.3%，禁食绵羊黏膜氧摄取中该部分占28.3%。
