Yamada K, Jordan R, Mori M, Speight P M
Department of Oral & Maxillofacial Surgery, Asahi University School of Dentistry, Japan.
Oral Dis. 1997 Jun;3(2):82-5. doi: 10.1111/j.1601-0825.1997.tb00017.x.
To evaluate the expression of E-cadherin, a calcium-dependent cell adhesion molecule, in a retrospective analysis of paraffin embedded tissue specimens of oral squamous cell carcinoma and relationship with the clinical TNM stage and histochemical differentiation.
Paraffin embedded tissue sections of normal oral mucosa (n = 6), oral squamous cell carcinoma (SCC, n = 18) and metastatic lymph nodes (n = 2) were immunostained by a three-stage streptoavidin-biotin immunoperoxidase method using monoclonal antibody. The TNM staging and histochemical grading were done according to the standard criteria.
Normal oral epithelium showed a strongly positive pericellular distribution of E-cadherin in basal, parabasal and spinous layers and no staining was observed in the parakeratinized and cornified layers. In well differentiated SCCs, the centrally located cells in tumour islands showed no staining, but peripheral basally located tumour cells showed positive staining. Poorly differentiated SCCs were devoid of staining. In moderately differentiated SCCs, the staining pattern was found to be one of the following three types: (1) a pattern similar to that of well differentiated SCC; (2) central cells of tumour aggregates were reactive but peripheral cells showed weak to negative reaction; and (3) all cells showed a negative reaction, resembling the poorly differentiated SCC. Fischer exact test showed a statistically significant correlation with loss of E-cadherin and grades of tumour differentiation as well as an advancing T and N stage.
The loss of E-cadherin may correlate with advancing T and N stages of the tumour and a poor tumour cell differentiation in oral squamous cell carcinomas.
通过对口腔鳞状细胞癌石蜡包埋组织标本进行回顾性分析，评估钙依赖性细胞黏附分子E-钙黏蛋白的表达情况，并探讨其与临床TNM分期及组织化学分化的关系。
采用单克隆抗体，通过三步链霉抗生物素蛋白-生物素免疫过氧化物酶法对正常口腔黏膜（n = 6）、口腔鳞状细胞癌（SCC，n = 18）和转移性淋巴结（n = 2）的石蜡包埋组织切片进行免疫染色。根据标准标准进行TNM分期和组织化学分级。
正常口腔上皮在基底、副基底和棘层显示E-钙黏蛋白在细胞周围呈强阳性分布，在角化不全层和角化层未观察到染色。在高分化SCC中，肿瘤岛中央的细胞无染色，但周边基底部位的肿瘤细胞呈阳性染色。低分化SCC无染色。在中分化SCC中，染色模式为以下三种类型之一：（1）与高分化SCC相似的模式；（2）肿瘤聚集体的中央细胞有反应，但周边细胞呈弱阳性至阴性反应；（3）所有细胞均呈阴性反应，类似于低分化SCC。费舍尔精确检验显示，E-钙黏蛋白的缺失与肿瘤分化程度以及T和N分期的进展在统计学上具有显著相关性。
E-钙黏蛋白的缺失可能与口腔鳞状细胞癌肿瘤的T和N分期进展以及肿瘤细胞分化不良相关。
