伴放线放线杆菌FDC Y4 S10操纵子部分片段的克隆与测序

Cloning and sequencing of part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4.

作者信息

Hayashida H, Hotokezaka H, Ohara N, Kimura M, Takagi O, Yamada T

机构信息

Department of Preventive Dentistry, Nagasaki University School of Dentistry, Japan.

出版信息

Oral Microbiol Immunol. 1997 Jun;12(3):174-7. doi: 10.1111/j.1399-302x.1997.tb00375.x.

DOI:10.1111/j.1399-302x.1997.tb00375.x

PMID:9467404

Abstract

We have cloned and sequenced the 5.2 kb EcoRI fragment that contained part of the S10 operon from Actinobacillus actinomycetemcomitans FDC Y4. The order of the ribosomal protein genes was identical to that of the S10 operon of Haemophilus influenzae and Escherichia coli. The deduced amino acid sequences of ribosomal proteins in this operon displayed significant homologies (65.3%-100%) to those of H. influenzae, E. coli, Yersinia enterocolitica and Yersinia pseudotuberculosis. Phylogenetic trees obtained for these ribosomal proteins were similar to that obtained for 16S rRNA.

摘要

我们已经克隆并测序了来自伴放线放线杆菌FDC Y4的包含S10操纵子部分的5.2 kb EcoRI片段。核糖体蛋白基因的顺序与流感嗜血杆菌和大肠杆菌的S10操纵子相同。该操纵子中核糖体蛋白的推导氨基酸序列与流感嗜血杆菌、大肠杆菌、小肠结肠炎耶尔森菌和假结核耶尔森菌的核糖体蛋白序列具有显著同源性（65.3%-100%）。从这些核糖体蛋白获得的系统发育树与从16S rRNA获得的系统发育树相似。