从人龈下菌斑分离出的一种口腔密螺旋体的基因特征分析。

Genetic characterization of an oral treponeme isolated from human subgingival plaque.

作者信息

Nakazawa F, Umemoto T, Namikawa I, Hoshino E

机构信息

Department of Oral Microbiology, School of Dentistry, Niigata University, Japan.

出版信息

Oral Microbiol Immunol. 1997 Jun;12(3):189-92. doi: 10.1111/j.1399-302x.1997.tb00378.x.

DOI:10.1111/j.1399-302x.1997.tb00378.x

PMID:9467407

Abstract

We examined the genetic characteristics of a human oral treponeme isolated from subgingival plaque of a patient with periodontal disease that could not be assigned to any of the previously described oral Treponema species. The guanine-plus-cytosine content of its DNA was 51 mole%. The levels of DNA-DNA relatedness of the organism to other Treponema species, including Treponema denticola, Treponema vincentii, Treponema socranskii, Treponema pallidum and Treponema phagedenis, were less than 30%. This study clearly demonstrated that the isolated treponeme could be completely distinct from the established oral Treponema species genetically. Also, the study indicated remarkable genetic heterogeneity among human oral Treponema species.

摘要

我们检测了从一名牙周病患者龈下菌斑中分离出的一种人类口腔密螺旋体的遗传特征，该密螺旋体无法归类到任何先前描述的口腔密螺旋体物种中。其DNA的鸟嘌呤加胞嘧啶含量为51摩尔%。该微生物与其他密螺旋体物种，包括齿垢密螺旋体、奋森密螺旋体、索氏密螺旋体、梅毒密螺旋体和溃蚀性密螺旋体的DNA-DNA相关性水平均低于30%。这项研究清楚地表明，分离出的密螺旋体在遗传上可能与已确定的口腔密螺旋体物种完全不同。此外，该研究还表明人类口腔密螺旋体物种之间存在显著的遗传异质性。