通过聚合酶链反应检测与人类牙周炎相关的索氏密螺旋体。

Detection of Treponema socranskii associated with human periodontitis by PCR.

作者信息

Sakamoto M, Takeuchi Y, Umeda M, Ishikawa I, Benno Y, Nakase T

机构信息

Japan Collection of Microorganisms, The Institute of Physical and Chemical Research (RIKEN), Wako, Saitama.

出版信息

Microbiol Immunol. 1999;43(5):485-90. doi: 10.1111/j.1348-0421.1999.tb02432.x.

DOI:10.1111/j.1348-0421.1999.tb02432.x

PMID:10449255

Abstract

A PCR technique was used to detect and identify Treponema socranskii associated with periodontitis. A species-specific forward primer was designed for a variable region within its 16S rRNA gene and was used in conjunction with a conserved reverse primer. This primer pair was tested for specificity against 44 oral bacterial strains. Sensitivity was determined using a serial dilution of T. socranskii cells. Amplification products were obtained from all T. socranskii strains tested, but not from other oral bacteria associated with periodontal disease. The detection limit of PCR was 5 T. socranskii cells per PCR. T. socranskii was detected by PCR in subgingival plaque and saliva samples from patients with periodontitis.

摘要

采用聚合酶链反应（PCR）技术检测和鉴定与牙周炎相关的索氏密螺旋体。针对其16S rRNA基因内的一个可变区设计了物种特异性正向引物，并与保守的反向引物联合使用。对该引物对针对44种口腔细菌菌株进行了特异性测试。通过对索氏密螺旋体细胞进行系列稀释来确定灵敏度。从所有测试的索氏密螺旋体菌株中获得了扩增产物，但未从与牙周疾病相关的其他口腔细菌中获得。PCR的检测限为每个PCR反应5个索氏密螺旋体细胞。通过PCR在牙周炎患者的龈下菌斑和唾液样本中检测到了索氏密螺旋体。