Elitsur Y, Hill I, Lichtman S N, Rosenberg A J
Pediatric Gastrointestinal Divisions of Marshall University, Huntington, West Virginia 25701-0195, USA.
Am J Gastroenterol. 1998 Feb;93(2):217-9. doi: 10.1111/j.1572-0241.1998.00217.x.
Rapid urease tests are reliable methods to diagnose Helicobacter pylori (HP) infection in the endoscopy suite. The PyloriTek test kit is a new rapid urease test that has the advantage of a 1-h final reading. The aim of this study was to compare the accuracy of PyloriTek and the test in the diagnosis of H. pylori infection in children.
Children from four different pediatric gastroenterology centers were recruited prospectively into the study. These children were >5 yr old and had an upper endoscopy procedure. Antral biopsies were examined for both rapid urease tests in the endoscopy suite, and others were sent for routine histological examination.
A total of 242 children were recruited into the study over approximately 1 yr. The concordance between PyloriTek and CLO test was 98% (238 of 242). Twenty-five children were positive for HP organisms by PyloriTek and CLO test, whereas four children were positive by PyloriTek but negative by CLO test. PyloriTek was comparable to CLO test for the diagnosis of HP organisms and HP-associated gastritis. Moreover, in 48% of the positive results, PyloriTek gave significantly faster results than CLO test.
We conclude that PyloriTek is an appropriate rapid urease test to use in children and may have an advantage over the CLO test because of its shorter reading time.
快速尿素酶试验是在内镜检查室诊断幽门螺杆菌（HP）感染的可靠方法。幽门螺杆菌检测试剂盒（PyloriTek）是一种新型快速尿素酶试验，其优点是最终读数时间为1小时。本研究的目的是比较PyloriTek与其他检测方法在诊断儿童幽门螺杆菌感染方面的准确性。
前瞻性招募来自四个不同儿科胃肠病学中心的儿童参与本研究。这些儿童年龄大于5岁，且接受了上消化道内镜检查。在内镜检查室对胃窦活检组织进行两种快速尿素酶试验检测，其他组织送去进行常规组织学检查。
在大约1年的时间里，共有242名儿童被纳入研究。PyloriTek与CLO试验的一致性为98%（242例中的238例）。25名儿童通过PyloriTek和CLO试验检测出HP阳性，而4名儿童通过PyloriTek检测为阳性，但通过CLO试验检测为阴性。在诊断HP感染和HP相关性胃炎方面，PyloriTek与CLO试验相当。此外，在48%的阳性结果中，PyloriTek得出结果的速度明显快于CLO试验。
我们得出结论，PyloriTek是一种适用于儿童的快速尿素酶试验，由于其读数时间较短，可能比CLO试验更具优势。
