Stirling C E
J Microsc. 1976 Mar;106(2):145-57. doi: 10.1111/j.1365-2818.1976.tb02397.x.
The distribution of salt transporting sites in epithelia is a problem of special interest to electrolyte metabolism. Autoradiography of freeze-dried plastic embedded tissue was used to localize 3H-ouabain in rabbit small intestine and renal medulla and gills of killifish. Resolution approaching theoretical limits for tritium at the light microscope level was achieved. The results of several tests are presented which demonstrate the resolution of the method and the specificity of the observed ouabain binding patterns. A large uptake of ouabain was found along the basolateral membranes of the enterocyte, thick ascending tubules and chloride cells (fish gill).
上皮细胞中盐转运位点的分布是电解质代谢特别感兴趣的一个问题。利用冻干塑料包埋组织的放射自显影技术，在兔小肠、肾髓质和鳉鱼鳃中定位³H-哇巴因。在光学显微镜水平上达到达到氡到了接近氚理论极限的分辨率。给出了几项测试结果，这些结果证明了该方法的分辨率以及所观察到的哇巴因结合模式的特异性。在肠上皮细胞、髓袢升支粗段和氯细胞（鱼鳃）的基底外侧膜上发现有大量的哇巴因摄取。
