Groves P, Finn B E, Kuźnicki J, Forsén S
Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Warsaw, Poland.
FEBS Lett. 1998 Jan 16;421(3):175-9. doi: 10.1016/s0014-5793(97)01535-4.
S100 proteins are a family of dimeric calcium-binding proteins implicated in several cancers and neurological diseases. Calbindin D9k is an unusual monomeric member of the S100 family. A calbindin D9k mutant containing a novel calcium-induced helix is characterized. Based on sequence comparison, this helix could be a component of other S100 proteins and a factor in target protein binding. The origin of structural differences between three reported apo S100 dimer structures is verified. We conclude that the differences are a result of modeling rather than a function of different target binding properties. A mechanism for target protein binding is suggested.
S100蛋白是一类二聚体钙结合蛋白，与多种癌症和神经疾病有关。钙结合蛋白D9k是S100家族中一种不同寻常的单体成员。对一种含有新型钙诱导螺旋的钙结合蛋白D9k突变体进行了表征。基于序列比较，这种螺旋可能是其他S100蛋白的一个组成部分以及靶蛋白结合的一个因素。验证了三种已报道的无配体S100二聚体结构之间结构差异的来源。我们得出结论，这些差异是建模的结果，而非不同靶标结合特性的作用。提出了一种靶蛋白结合机制。
