Takagi A, Imai A, Horibe S, Ohno T, Tamaya T
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Tsukasamachi, Gifu 500, Japan.
Oncol Rep. 1998 Mar-Apr;5(2):377-80. doi: 10.3892/or.5.2.377.
Interaction of Fas and its ligand plays an important role in the cell death by means of apoptosis in a variety of cell types. We recently demonstrated the frequent expression of Fas in female reproductive tract tumors, which prompted us to examine the local production of Fas ligand by the Fas-bearing tumors. Human endometrial and ovarian carcinomas, surgically removed, were studied. No expression of Fas ligand messenger ribonucleic acid (mRNA) as assessed by reverse transcription-polymerase chain reaction using oligonucleotide primers according to published sequences were observed; Fas ligand mRNA was detected in lymphocytes. Immunoblotting of membrane proteins with the specific antibody failed to demonstrate any substantial level of immunoreactive Fas ligand in these tumors. The results for both mRNA expression and immunoreactivity suggest that human ovarian and endometrial carcinomas lack the Fas ligand. The demonstration of absence of Fas ligand expression raises the possibility that the stimulated proliferation of the Fas ligand-absent tumors may occurs by release of Fas-Fas ligand system-associated apoptosis.
Fas与其配体的相互作用在多种细胞类型通过凋亡方式导致的细胞死亡中发挥重要作用。我们最近证实Fas在女性生殖道肿瘤中频繁表达，这促使我们研究携带Fas的肿瘤局部Fas配体的产生情况。我们对手术切除的人子宫内膜癌和卵巢癌进行了研究。使用根据已发表序列设计的寡核苷酸引物，通过逆转录-聚合酶链反应评估，未观察到Fas配体信使核糖核酸（mRNA）的表达；在淋巴细胞中检测到Fas配体mRNA。用特异性抗体对膜蛋白进行免疫印迹未能在这些肿瘤中显示出任何显著水平的免疫反应性Fas配体。mRNA表达和免疫反应性的结果均表明人卵巢癌和子宫内膜癌缺乏Fas配体。Fas配体表达缺失的证实增加了一种可能性，即缺乏Fas配体的肿瘤的增殖可能是通过Fas-Fas配体系统相关凋亡的释放而发生的。
