Murata T, Akagi K, Kimura H, Nasu R, Imamura M, Tanaka Y
Department of Radiology, Kansai Medical University, 10-15 Fumizonochou, Moriguchi City, Osaka, 570, Japan.
Oncol Rep. 1998 Mar-Apr;5(2):385-8. doi: 10.3892/or.5.2.385.
Irradiation, dose-dependently, increases the percentage of cells in the G2 + M phase and the duration of cell cycle arrest, reflected by changes in cell kinetics in the first 48 h. Cells in the G2 + M phase are considered radiosensitive but little is known about their proliferative ability. We studied proliferative ability of irradiated G2 phase-arrested cells following flow cytometric cell sorting using fluorescent Hoechst 33342 staining. Although proliferative ability of non-irradiated cells was not different between G1 and G2 + M phases, cells arrested in G2 + M after irradiation, especially at higher doses, showed less ability than cells in G1. Proliferative ability also correlated well with of G2 block duration.
照射剂量依赖性地增加了处于G2 + M期的细胞百分比以及细胞周期停滞的持续时间，这在前48小时的细胞动力学变化中得到体现。处于G2 + M期的细胞被认为对辐射敏感，但对其增殖能力了解甚少。我们使用荧光Hoechst 33342染色通过流式细胞术细胞分选研究了受照射的G2期停滞细胞的增殖能力。尽管未受照射的细胞在G1期和G2 + M期之间的增殖能力没有差异，但照射后停滞在G2 + M期的细胞，尤其是在较高剂量下，其增殖能力比G1期的细胞弱。增殖能力也与G2期阻滞持续时间密切相关。
