Maschler I, Salzberger M, Finkelstein M
Acta Endocrinol (Copenh). 1976 Jun;82(2):366-79. doi: 10.1530/acta.0.0820366.
When ovarian mitochondria from patients with polycystic ovary syndrome (POS) were incubated with [7-3H]17alpha-hydroxypregnenolone and [4-14C]-17alpha-hydroxyprogesterone, 11beta-hydroxylated metabolites were obtained. The mitochondria, prepared from pooled, frozen, polycystic ovarian tissue of 5 patients, converted [7-3H]17alpha-hydroxypregnenolone to 3beta, 11beta, 17alpha--trihydroxy-5-pregnen-20-one (yield 0.065%) and to 3beta, 17alpha-dihydroxy-5-pregnene-11,20-dione (0.22%), while [4-14C]17alpha-hydroxyprogesterone was converted to 21-deoxycortisol (0.1%). Incubation of mitochondria, prepared from 4 pooled samples of frozen, normal ovarian tissue, yielded no evidence of 11beta-hydroxylation of either of the substrates. Mitochondria obtained from fresh, polycystic ovarian tissue of a single patient with POS converted [7-3H]17alpha-hydroxypregnenolone to 3beta,17alpha-dihydroxy-5-pregnene-11,20-dione (2.1%) and [4-14C]17alpha-hydroxyprogesterone to 21-deoxycortisol (0.1%). When the same mitochondrial preparation was incubated simultaneously with [7-3H]17alpha-hydroxypregnenolone and [4-14C]11-deoxycortisol, it converted 17alpha-hydroxypregnenolone to 3beta,17alpha-dihydroxy-5-pregnene-11,20-dione (1.9%), but no 11beta-hydroxylated derivatives of 11-deoxycortisol were found. These results demonstrate that ovaries of patients with POS contain an 11beta-hydroxylase active towards C-21-deoxysteroids but inert to C-21-hydroxysteroids such as 11-deoxycortisol.
将多囊卵巢综合征（POS）患者的卵巢线粒体与[7-³H]17α-羟孕烯醇酮和[4-¹⁴C]-17α-羟孕酮一起孵育时，可得到11β-羟基化代谢产物。从5例患者的冷冻多囊卵巢组织混合样本中制备的线粒体，将[7-³H]17α-羟孕烯醇酮转化为3β,11β,17α-三羟基-5-孕烯-20-酮（产率0.065%）和3β,17α-二羟基-5-孕烯-11,20-二酮（0.22%），而[4-¹⁴C]17α-羟孕酮则转化为21-脱氧皮质醇（0.1%）。对从4个冷冻正常卵巢组织混合样本中制备的线粒体进行孵育，未发现两种底物有11β-羟基化的证据。从一名患有POS的患者新鲜多囊卵巢组织中获得的线粒体，将[7-³H]17α-羟孕烯醇酮转化为3β,17α-二羟基-5-孕烯-11,20-二酮（2.1%），并将[4-¹⁴C]17α-羟孕酮转化为21-脱氧皮质醇（0.1%）。当将相同的线粒体制剂与[7-³H]17α-羟孕烯醇酮和[4-¹⁴C]11-脱氧皮质醇同时孵育时，它将17α-羟孕烯醇酮转化为3β,17α-二羟基-5-孕烯-11,20-二酮（1.9%），但未发现11-脱氧皮质醇的11β-羟基化衍生物。这些结果表明，POS患者的卵巢含有一种对C-21-脱氧类固醇有活性但对C-21-羟基类固醇（如11-脱氧皮质醇）无活性的11β-羟化酶。
